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Rats show unimpaired learning within minutes after recovery from single bolus propofol anesthesia

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Abstract

Purpose

To examine the learning ability of rats shortly after recovery from a bolus dose of propofol by assessing learning on a swim-to-platform task. Also, muscarinic blockade was used as a pharmacological test of whether learning shortly after propofol anesthesia resembles normal learning.

Methods

Propofol anesthetized rats (15–20 mg · kg−1 iv) were trained on a swim-to-platform task five to seven minutes after recovering from surgical anesthesia and tested two to three hours later. In addition, the muscarinic antagonist scopolamine hydrobromide (5 mg · kg−1 sc) was given to a subgroup of rats before testing. During 10 trials, the number of times a given rat took 10 sec or longer to locate and climb onto a visible platform was tabulated and counted as errors.

Results

When trained shortly after recovery from the anesthetic, propofol anesthetized rats made 3.2 ± 0.4 compared with 1.0 ± 0.1 errors in controls (P < 0.0001). Two to three hours later both groups performed equally well. Rats trained after propofol anesthesia and given scopolamine before testing made 0.7 ± 0.5 errors and performed as well as normal controls, 1.2 ± 0.2 errors when subjected to the same procedures without propofol anesthesia, and better than scopolamine-treated untrained rats, 5.5 ± 0.7 errors, (P < 0.05).

Conclusion

Training five to seven minutes after recovery from propofol anesthesia resulted in normal retention of the swim-to-platform task. It also produced the same resistance to the disruptive effects of scopolamine as did training in rats that were not anesthetized. Thus, the ability to learn recovers rapidly after propofol anesthesia induced by a single intravenous bolus dose.

Résumé

Objectif

Examiner la capacité d’apprendre des rats, peu après la récupération d’une anesthésie avec une dose bolus de propofol, en évaluant comment ils apprennent à nager vers une plate-forme. De plus, utiliser le blocage muscarinique en qualité de test pharmacologique de l’apprentissage, et voir si c’est comparable à un apprentissage normal.

Méthode

Des rats ayant reçu une anesthésie au propofol (15–20 mg · kg−1 iv) ont été entraînés à nager vers une plateforme, cinq à sept minutes après la récupération de l’anesthésie et ont été testés de nouveau deux à trois heures plus tard. La scopolamine, antagoniste muscarinique, a été administrée (5 mg · kg−1sc) à un sous-groupe de rats avant les essais. Pendant les 10 essais, le nombre de fois qu’un rat donné prenait 10 s ou plus pour atteindre une plate-forme visible et y grimper ont été considérées comme des erreurs.

Résultats

Les rats entraînés peu après la récupération de l’anesthésie au propofol ont fait 3.2 ± 0.4 erreurs et les rats témoins, 1.0 ± 0.1 erreur (P < 0.0001). Deux ou trois heures plus tard, les performances étaient égales pour les rats des deux groupes. Les rats entraînés après l’anesthésie et qui ont reçu de la scopolamine avant les essais ont fait 0.7 ± 0.5 erreur, faisant aussi bien que les rats témoins, 1.2 ± 0.2 erreur, soumis aux mêmes épreuves sans anesthésie au propofol, et mieux que les rats non entraînés mais traités à la scopolamine, 5.5 ± 0.7 erreurs (P < 0.05).

Conclusion

Lentraînement, cinq à sept minutes après la récupération de l’anesthésie au propofol, a permis une rétention normale de l’apprentissage qui consistait à nager vers une plate-forme, Cela a produit aussi la même résistance aux effets perturbateurs de la scopolamine que l’entraînement des rats non anesthésiés. Ainsi, la capacité d’apprendre est rapidement récupérée après l’anesthésie au propofol induite avec une dose unique en bolus intraveineux.

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Author information

Correspondence to Christopher G. Engeland.

Additional information

Supported by an operating grant to C.H. Vanderwolf from NSERC.

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Engeland, C.G., Vanderwolf, C.H. & Gelb, A.W. Rats show unimpaired learning within minutes after recovery from single bolus propofol anesthesia. Can J Anesth 46, 586 (1999). https://doi.org/10.1007/BF03013552

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Keywords

  • Scopolamine
  • Training Trial
  • Retention Testing
  • Disruptive Effect
  • Behav Brain