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Superoxide radical generation and histopathological changes in hippocampal CA1 after ischaemia/ reperfusion in gerbils

  • Shigeki Yamaguchi
  • Hiromaru Ogata
  • Shinsuke Hamaguchi
  • Toshimitsu Kitajima
Laboratory Reports

Abstract

Purpose

We investigated the relationship between the generation of Superoxide radicals and histopathological changes on delayed neuronal death in the hippocampal CA1 subfield.

Methods

Seventy gerbils were randomly assigned to two groups, a sham group and an ischaemia/reperfusion (I/R) group. In the I/R group, transient forebrain ischaemia was induced by occluding the bilateral common carotid arteries for four minutes. The cerebrum was removed after reperfusion at intervals of one minute, six, twelve and twenty-four hr and at three, five and seven days. Each forebrain was cut into two portions including the hippocampus. The quantity of Superoxide radicals was measured by using chemiluminescence, and histopathological changes in the hippocampal CAI subfield were examined.

Results

In the I/R group, Superoxide radicals increased on the 3rd and 5th days compared with the sham group (16.1 ±3.4vs3.2± 1.0 on the third day (P < 0.0001 ); 10.9 ± 1.9 vs 3.3 ± 0.8 on the fifth day (P < 0.0001)). In the I/R group, the pyramidal cells were atrophic and pycnotic; vacuolation, and structural disruption of the radial striated zone were observed from the third through the seventh day. In the sham group, these changes were not observed. There were differences of degenerative ratios in the pyramidal cells between the two groups from the third to seventh days (5.6 ± 2.0 vs 80.9 ± 3.3 on the third day (P < 0.05); 6.9 ± 0.4 vs 93.6 ± 2.4 on the fifth day (P < 0.05); 6.2 ± 1.5 vs 95.0 ± 1.3 on the seventh day (P < 0.05)).

Conclusion

There is a correlation between the generation of Superoxide radicals and histopathological changes of the pyramidal cells in the hippocampal CAI subfield.

Keywords

Neuronal Death Sham Group Superoxide Radical Pyramidal Cell Histopathological Change 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Résumé

Objectif

Investiguer la relation entre la production des radicaux superoxydes et les changements histopathologiques sur le décès neuronal retardé dans le champ hippocampique CAL.

Méthodes

Soixante-dix gerbilles ont été aléatoirement réparties en 2 groupes, un groupe contrôle et un groupe ischémie/reperfusion (I/R). Dans le groupe I/R, une ischémie transitoire du prosencéphale était induite par l’occlusion bilatérale des carotides communes pour quatre minutes. Après reperfusion, le cerveau était retiré de l’animal après une minute de même qu’à six, douze et vingt-quatre heures ainsi qu’à trois, cinq et sept jours. Chaque prosencéphale était coupé en deux parties incluant l’hippocampe. La quantité de radicaux superoxydes était mesurée par chemiluminescence et les changements histopathologiques dans le champ hippocampique CAI étaient observés.

Résultats

Dans le groupe I/R, les radicaux superoxydes ont augmenté aux jours 3 et 5 comparativement au groupe témoin (16,1 ±3,4 vs 3,2± 1,0 au jour 3 (P< 0,000l); 10,9 ± 1,9 vs 3,3 ± 0,8 au jour 5 (P< 0,000l)). Dans le groupe I/R, les cellules pyramidales étaient atrophiques et picnotiques; du 3e au 7e jour, on a observé de la vacuolisation et de la destruction structurale de la zone striée radiaire, et ces changements n’ont pas été retrouvés dans le groupe témoin. On a observé des différences dans le pourcentage dégénératif des cellules pyramidales entre les deux groupes à partir du jour 3 au jour 7 (5,6 ± 2,0 vs 80,9 ± 3,3 au jour 3 (P< 0,05); 6,9 ± 0,4 vs 93,6 ± 2,4 au jour 5 (P< 0,05); 6,2 ± 1,5vs 95,0 ± 1,3 au jour 7 (P< 0,05)).

Conclusion

Il y a une corrélation entre la production de radicaux superoxydes et les changements histopathologiques des cellules pyramidales du champ CAI de l’hippocampe.

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Copyright information

© Canadian Anesthesiologists 1998

Authors and Affiliations

  • Shigeki Yamaguchi
    • 1
  • Hiromaru Ogata
    • 1
  • Shinsuke Hamaguchi
    • 1
  • Toshimitsu Kitajima
    • 1
  1. 1.Department of AnaesthesiologyDokkyo University, School of MedicineMibu TochigiJapan

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