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Decreased hypothalamic prostaglandin D2 and prostaglandin E2 contents during isoflurane anaesthesia in rats

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This study was undertaken to evaluate the effect of isoflurane anaesthesia on the hypothalamic contents of both prostaglandin D2 and E2 which affect the sleep-wakefulness cycle. Sixty-three Wistar rats were divided into three equal groups, control, iso-flurane and recovery groups. Twenty-one rats of the control did not receive isoflurane. In the other groups 21 rats received isoflurane 2% far 30 min and 21 received isoflurane 2% for 30 min and were allowed to recover their usual behaviours, including righting reflex, spontaneously. The hypothalamus was removed and the contents of PGD2 and PGE2 were measured by enzyme immunoassay. The PGD2 content in the hypothalamus was 397.9 ± 226.0 pg· g− 1 for the control group, 134.2 ± 41.2 pg· g−1 for the isoflurane group and 269.1 ± 124.6 pg · g−1 for the recovery group, respectively. The hypothalamic PGE2 contents were 381.4 ± 139.0 pg · g−1 for the control group, 183.3 ± 26.4 pg · g−1 for the isoflurane group and 312.2 ± 96.0 pg · g−1 for the recovery group, respectively. The hypothalamic PGD2 and PGE2 contents in the isoflurane group were lower (P < 0.05) than those in the control and recovery groups, while both the PGD2 and PGE2 contents of the control and the recovery groups were similar. We conclude that decreased hypothalamic PGD2 and PGE2 contents may be related to some manifestations of general anaesthesia with isoflurane.


Cette étude vise à évaluer l’effet de l’isoflurane sur la teneur en prostaglandines D2 et E2 de l’hypothalamus, substances qui affectent le cycle du sommeil et de l’éveil. Soixante-six rats Wistar sont repartis en trois groupes identiques: contrôle, iso-flurane et récupération. Vingt-et-un rats du groupe contrôle ne reçoivent pas d’isoflurane. Dans les deux autres groupes, 21 rats reçoivent de l’isoflurane 2% pendant 30 min et 21 rats reçoivent de l’isoflurane pendant 30 min suivi de la récupération spontanée de leur comportement usuel incluant le réflexe de redressement. L’hypothalamus est alors isolé et le contenu en PGD2 et PGE2 est mesuré par épreuve immunologique. La teneur de l’hypothalamus en PGD2 est respectivement 397,9 ± 226,0 pg · g−1 dans le group contrôle, 134,2 ± 41,2 pg · g−1 dans le groupe isoflurane et de 269,1 ± 124,6 pg· g−1 dans le groupe récupération. Le contenu hypothalamique de PGE2 est respectivement de 381,4 ± 139,0 pg · g−1 pour le groupe contrôle, 183,3 ± 26,4 pg · g−1 pour le groupe isoflurane, et de 312,2 ± 96,0 pg · g−1 pour le groupe récupération. La teneur de hypothalamus en PGD2 et en PGE2 du groupe isoflurane est plus basse (P < 0,05) que celle du groupe contrôle et du groupe récupération. Les auteurs concluent que la diminution du contenu hypothalamique de PGD2 et de PGE2 pourrait être un effet de l’anesthésie à lisoflurane.


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Correspondence to Akitomo Matsuki.

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Sato, T., Araki, I., Kushikata, T. et al. Decreased hypothalamic prostaglandin D2 and prostaglandin E2 contents during isoflurane anaesthesia in rats. Can J Anaesth 42, 1031 (1995). https://doi.org/10.1007/BF03011078

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Key words

  • anaesthesia: depth
  • anaesthetics, volatile: isoflurane
  • hormones: prostaglandin D2, E2