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Determination of blood halothane levels by gas chromatography

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Summary

A method of isolation by distillation of halothane from blood and its subsequent Chromatographic detection and quantitation have been described. The method is simple, rapid, and inexpensive. It lends itself well to incorporation into anaesthetic investigations when blood (and tissue) anaesthetic levels must be obtained In serial manner.

The accuracy of the method, based on tests of reproducibility and recovery, is within ±5 per cent. These data are not of a selected nature and represent a testing of the isolation and Chromatographic techniques for quantitative analytical adequacy.

Some data obtained from animal experiments are presented. Detailed correlation of these data with cardiovascular and acid-base investigations will appear elsewhere.13

Résumé

Nous avons décrit une méthode d’identification de l’halothane par sa distillation du sang et sa détection chromatographique subséquente et son titrage. La méthode est simple, rapide, peu coûteuse. Elle peut être entreprise aussi bien avec d’autres investigations anesthésiques quand les niveaux d’anesthésie dans le sang et dans les tissus doivent être obtenus en série.

La précision de cette méthode basée sur des tests de reproduction et de réveil est de +5 pour cent. Ces valeurs ne sont pas de nature sélective et elles représentent un test d’identification et de technique chromatographique pour une analyse quantitative adéquate.

Nous avons présenté quelques données obtenjues des expériences sur des animaux. Nous publierons ailleurs la corrélation detaillée entre ces notions et des investigations sur l’équilibre acide-base et sur le système cardiovasculaire.

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Author information

Correspondence to Richard H.Gadsden or K. Benjamin or H. Risinger or Ervin E. Bagwell.

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H.Gadsden, R., Benjamin, K., Risinger, H. et al. Determination of blood halothane levels by gas chromatography. Can. Anaes. Soc. J. 12, 90–98 (1965). https://doi.org/10.1007/BF03004081

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Keywords

  • Halothane
  • Potassium Carbonate
  • Methoxyflurane
  • Sodium Tungstate
  • Fluothane