Rendiconti Lincei

, Volume 3, Issue 4, pp 375–381 | Cite as

Determination of the sequence and study of the expression of a gene for the heat shock protein (hsp) 70 of the sea urchin Paracentrotus lividus

  • Maria Grazia Ferraro
  • Giuseppe Scardina
  • Domenico Cascino
  • Gabriella Sconzo
  • Giovanni Giudice
Biologia molecolare


The sequence of a gene coding for the hsp70 isolated from a P.lividus genomic library is reported. This gene is interrupted by a long intron at 183 nt downstream the translation start codon. Its promoter region contains three heat shock elements, thus indicating that the gene is an inducible one. Northern blot analysis shows that this gene is transcribed following heat shock and that its transcription products bind to ribosomes.

Key words

Heat shock gene Sea urchin Embryos 

Determinazione delta sequenza nucleotidica e studio dell’espressione di un gene per la proteina da shock da calore di 70 kDa del riccio di mare Paracentrotus lividus


Si riporta la sequenza nucleotidica di un gene per la proteina da shock da calore di 70 kDa isolato da una genoteca di riccio di mareParacentrotus lividus. Quesro gene è interrotto da un lungo introne a 183 nucleotidi a valle della tripletta per l’inizio della traduzione. La sua regione del promotore contiene tre elementiheat shock, il ehe indica ehe trattasi di un gene inducibile. Un’analisi perNorthern blot mostra ehe il gene è trascritto a seguito di shock da calore e che i prodotti della sua trascrizione si legano ai ribosomi.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. [1]
    S. Lindquist -E. A. Craig,The heat shock proteins. Annu. Rev. Genet., 22, 1988, 631–677.CrossRefGoogle Scholar
  2. [2]
    M. L. Pardue,The heat shock response in biology and human disease: a meeting review. Genes Develop., 2, 1988, 783–785.CrossRefGoogle Scholar
  3. [3]
    R. I. Morimoto - A. Tissieres - C. Georgopoulos (eds.),Stress proteins in biology and medicine. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y., 1990.Google Scholar
  4. [4]
    B. Maresca -S. Lindquist (eds.),Heat Shock. Springer-Verlag, Berlin 1991.Google Scholar
  5. [5]
    J. R. Ellis -S. M. Van der Vies,Molecular chaperons. Annu. Rev. Biochem., 60, 1991, 321–347.CrossRefGoogle Scholar
  6. [6]
    W. J. Welch,The role of heat-shock proteins as molecular chaperons. Curr. Op. Cell Biol, 3, 1991, 1033–1038.CrossRefGoogle Scholar
  7. [7]
    G. Gil’Dice,Heat shock proteins in sea urchin embryos. Develop. Growth Diff., 31, 1989, 103–106.CrossRefGoogle Scholar
  8. [8]
    M. La Rosa -G. Sconzo -G. Giudice -M. C. Roccheri -M. Dr Carlo,Sequence of a sea urchin hsp70gene and its 5’ flanking region. Gene, 96, 1990, 295–300.CrossRefGoogle Scholar
  9. [9]
    F. Sénger -S. Nicklen -A. R. Coulson,DNA sequencing with chain-terminating inhibitors. Proc. Nad. Acad. Sci. USA, 74, 1977, 5463–5467.CrossRefGoogle Scholar
  10. [10]
    J. Samhkook -E. F. Fritsch -T. Maniatis,Molecular cloning: A laboratory manual. 2nd ed., Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y. 1989.Google Scholar
  11. [11]
    J. Winter -R. Wright -N. Duck -C. Gasser -R. Fraley -D. Shah,The inhibition of petunia hsp7OmRNA processing during CdCl 2 stress. Mol. Gen. Genet., 211, 1988, 315–319.CrossRefGoogle Scholar
  12. [12]
    J C. Hunt -R. I. Morimoto,Conserved features of eukaryotic hsp70 genes revealed by comparison with the nucleotule sequence of human hsp70. Proc. Nad. Acad. Sci. USA, 82, 1985, 6455–6459.CrossRefGoogle Scholar
  13. [13]
    P. K. Sorger -H. R. B. Pelham,Cloning and expression of a gene encoding hsc73,the major hsp70-like protein in unstressed rat cells. EMBO J., 6, 1987, 993–998.Google Scholar
  14. [14]
    T. P. Snitch -M. F. P. Heschl -D. L. Baillie,The Caenorhabditis elegans hsp70gene family: a molecular genetic characterization. Gene, 64, 1988, 241–255.CrossRefGoogle Scholar
  15. [15]
    D. E. Rochester -J. A. Winer -D. M. Shah,The structure and expression of maize genes encoding the major heat shock protein, hsp70. EMBO J., 5, 1986, 451–458.Google Scholar
  16. [16]
    J. Bardwell -E. A. Craig,Major heat shock gene a Drosophila and Escherichia coli heat inducible dnak gene are homologous. Proc. Natl. Acad. Sci. USA, 81, 1984, 848–852.CrossRefGoogle Scholar
  17. [17]
    L. A. Perkins -J. S. Doctor -K. Zhang -L. Stinson -N. Perrimon -E. A. Craig,Molecular and developmental characterization of the heat shock cognate 4 gene of Drosophila melanogaster. Mol. Cell. Biol., 10, 1990, 3232–3238.Google Scholar
  18. [18]
    B. J. Wu -G. T. Williams -R. I. Morimoto,Detection of three protein binding sites in the serumregulated promoter of the human gene encoding the 70 kDa heat shock protein. Proc. Natl. Acad. Sci. USA, 84, 1987, 2203–2207.CrossRefGoogle Scholar

Copyright information

© Accademia nazionale dei Lincei 1992

Authors and Affiliations

  • Maria Grazia Ferraro
    • 1
  • Giuseppe Scardina
    • 2
  • Domenico Cascino
    • 1
  • Gabriella Sconzo
    • 1
  • Giovanni Giudice
    • 1
    • 2
  1. 1.Dipartimento di Biologia Cellulare e dello SviluppoUniversità degli StudiPalermo
  2. 2.Istituto di Biologia dello Sviluppo - C.N.R. - Via ArchiratiPalermo

Personalised recommendations