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Rendiconti Lincei

, Volume 3, Issue 4, pp 375–381 | Cite as

Determination of the sequence and study of the expression of a gene for the heat shock protein (hsp) 70 of the sea urchin Paracentrotus lividus

  • Maria Grazia Ferraro
  • Giuseppe Scardina
  • Domenico Cascino
  • Gabriella Sconzo
  • Giovanni Giudice
Biologia molecolare
  • 18 Downloads

Abstract

The sequence of a gene coding for the hsp70 isolated from a P.lividus genomic library is reported. This gene is interrupted by a long intron at 183 nt downstream the translation start codon. Its promoter region contains three heat shock elements, thus indicating that the gene is an inducible one. Northern blot analysis shows that this gene is transcribed following heat shock and that its transcription products bind to ribosomes.

Key words

Heat shock gene Sea urchin Embryos 

Determinazione delta sequenza nucleotidica e studio dell’espressione di un gene per la proteina da shock da calore di 70 kDa del riccio di mare Paracentrotus lividus

Riassunto

Si riporta la sequenza nucleotidica di un gene per la proteina da shock da calore di 70 kDa isolato da una genoteca di riccio di mareParacentrotus lividus. Quesro gene è interrotto da un lungo introne a 183 nucleotidi a valle della tripletta per l’inizio della traduzione. La sua regione del promotore contiene tre elementiheat shock, il ehe indica ehe trattasi di un gene inducibile. Un’analisi perNorthern blot mostra ehe il gene è trascritto a seguito di shock da calore e che i prodotti della sua trascrizione si legano ai ribosomi.

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Copyright information

© Accademia nazionale dei Lincei 1992

Authors and Affiliations

  • Maria Grazia Ferraro
    • 1
  • Giuseppe Scardina
    • 2
  • Domenico Cascino
    • 1
  • Gabriella Sconzo
    • 1
  • Giovanni Giudice
    • 1
    • 2
  1. 1.Dipartimento di Biologia Cellulare e dello SviluppoUniversità degli StudiPalermo
  2. 2.Istituto di Biologia dello Sviluppo - C.N.R. - Via ArchiratiPalermo

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