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Immunocytochemical evaluation abl-Gene products in Leukemic Cell Lines

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We raised monoclonal antibody (MAb) against a synthetic oligopeptide corresponding to a portion of the predictedv-abl protein sequence (379–390). This MAb reacted with all of theabl-gene products (pl45c-abl, pl50c-abl and 210bcr-abl fused protein) and was not specific for any one of them. Immunocytochemically, we investigated the expression and localization of theabl-gene products in various leukemic cell lines. Positive immunoreactions were observed in Ph1 positive leukemic cell lines (K562 and KU-812) and erythro-leukemic cell lines (HEL and K3D) and were located on the cell membrane. Electron microscopically, a different distribution pattern was observed among the cell lines: linear and almost even in Ph1 positive leukemic cell lines, whereas spotted or budding-like in erythroleukemic cell lines. Ph1 translocation produces p210nbcr-abl fused protein with not only altered autophosphorylation activities but also altered subcellular distribution patterns.

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Correspondence to Sohei Kitazawa or Sakan Maeda or Taketoshi Sugiyama.

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Kitazawa, S., Maeda, S. & Sugiyama, T. Immunocytochemical evaluation abl-Gene products in Leukemic Cell Lines. Med. Oncolo. & Tumor Pharmacother. 7, 35–41 (1990).

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Key words

  • Leukemia
  • Oncogene
  • c-abl
  • Monoclonal antibody
  • Immunocytochemistry