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125I-iomazenil-benzodiazepine receptor binding during psychological stress in rats

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Abstract

Objective: We investigated the changes in125I-iomazenil (125I-IMZ) benzodiazepine receptor (BZR) binding with psychological stress in a rat model.Methods: Six male Wistar rats were placed under psychological stress for 1 hour by using a communication box. No physical stress was not received. 1.85 MBq of125I-IMZ was injected into the lateral tail vein and the rat was killed 3 hours later. Twenty-micrometer-thick sections of the brain were collected and % injected dose per body weight (%ID/BW) of eleven regions (frontal, parietal, temporal, occipital cortices, caudate putamen, accumubens nuclei, globus pallidus, amygdala, thalamus, hippocampus and hypothalamus) were calculated by autoradiography. The %ID/BW of rats which were placed under psychological stress was compared with that of 6 control rats.Results: The %ID/BW of rats which were placed under psychological stress diffusely tended to show a reduction in125I-IMZ-BZR binding. A significant decrease in BZR binding was observed in the hippocampus of the rats which were placed under psychological stress.Conclusion:125I-IMZ-BZR binding tended to decrease throughout the brain.

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Correspondence to Nobuyoshi Fukumitsu.

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Fukumitsu, N., Tsuchida, D., Ogi, S. et al. 125I-iomazenil-benzodiazepine receptor binding during psychological stress in rats. Ann Nucl Med 16, 231–235 (2002). https://doi.org/10.1007/BF02996307

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Key words

  • 125I-iomazenil
  • benzodiazepine receptor
  • psychological stress
  • rat
  • communication box