Springer Nature is making SARS-CoV-2 and COVID-19 research free. View research | View latest news | Sign up for updates

Application of polyacrylamide gel electrophoresis/neutron activation analysis for protein quantification


A combination of two methods, polyacrylamide gel electrophoresis (PAGE) and neutron activation analysis (NAA), has been applied to solutions containing phosphoproteins for the purpose of protein quantification. The proteins were separated by molecular weight using PAGE, and then the whole gel was activated by neutron bombardment. Densitometric measurements of the developed bands from32P, taken from autoradiographs of the activated gels, resulted in quantification of the phosphorus, and then the related protein. This PAGE/NAA method was applied to several phosphoprotein-containing materials, including commercial milk products and reference materials, i.e., IAEA A-11, milk powder, and SRM 1845, Cholesterol in Egg Powder.

This is a preview of subscription content, log in to check access.


  1. 1.

    C. K. Jayawickreme and A. Chatt,J. Radioanal. Nucl. Chem. 124, 257 (1988).

  2. 2.

    K. S. Kwok, R. M. Snapka, J. A. Bernard, O. K. Harling, and A. Varshavsky,Trans. Am. Nucl. Soc. 49, 157 (1985).

  3. 3.

    Z. Szökefalvi-Nagy, I. Demeter, C. Bagyink, and K. L. Kovács,Nucl. Instr. Meth. Phys. Res. B22, 156 (1987).

  4. 4.

    S. F. Stone, R. Zeisler, and D. K. Hancock,J. Radioanal. Nucl. Chem. 112, 95 (1987)

  5. 5.

    S. F. Stone, R. Zeisler, and G. E. Gordon,Trace Element Analytical Chemistry in Medicine and Biology, vol. 5, P. Brätter and P. Schramel, eds., Walter de Guyter, Berlin-New York, 1988, p. 157.

  6. 6.

    U. K. Laemmli,Nature 227, 680 (1970).

  7. 7.

    J. Basch, F. W. Douglas, L. G. Procino, V. H. Holsinger, and H. M. Farrell,J. Dairy Sci,68, 23 (1985).

  8. 8.

    K. Olden and K. M. Yamada,Anal. Biochem. 78, 483 (1977).

  9. 9.

    K. F. NG Kwai-Lang and E. M. Kroeker,J. Dairy Sci 67, 3052 (1984).

  10. 10.

    B. Ridadeau Dumas,Biochim. Biophys. Acta 168, 274 (1968).

  11. 11.

    M. Ramos, R. M. Sanchez, and A. Olano,Chem. Milkrobiol. Technol. Lebensm. 9, 24 (1985).

  12. 12.

    G. D. Rolleri, B. L. Larson, and R. W. Touchberry,J. Dairy Sci. 39, 1683 (1956).

Download references

Author information

Correspondence to Susan F. Stone.

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Stone, S.F., Zeisler, R. & Gordon, G.E. Application of polyacrylamide gel electrophoresis/neutron activation analysis for protein quantification. Biol Trace Elem Res 26, 85–91 (1990).

Download citation

Index Entries

  • Casein
  • milk
  • milk products
  • neutron activation analysis (NAA)
  • phosphoprotein
  • phosvitin
  • polyacrylamide gel electrophoresis (PAGE)
  • protein
  • protein quantitation