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Application of polyacrylamide gel electrophoresis/neutron activation analysis for protein quantification

Abstract

A combination of two methods, polyacrylamide gel electrophoresis (PAGE) and neutron activation analysis (NAA), has been applied to solutions containing phosphoproteins for the purpose of protein quantification. The proteins were separated by molecular weight using PAGE, and then the whole gel was activated by neutron bombardment. Densitometric measurements of the developed bands from32P, taken from autoradiographs of the activated gels, resulted in quantification of the phosphorus, and then the related protein. This PAGE/NAA method was applied to several phosphoprotein-containing materials, including commercial milk products and reference materials, i.e., IAEA A-11, milk powder, and SRM 1845, Cholesterol in Egg Powder.

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Correspondence to Susan F. Stone.

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Stone, S.F., Zeisler, R. & Gordon, G.E. Application of polyacrylamide gel electrophoresis/neutron activation analysis for protein quantification. Biol Trace Elem Res 26, 85–91 (1990). https://doi.org/10.1007/BF02992661

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Index Entries

  • Casein
  • milk
  • milk products
  • neutron activation analysis (NAA)
  • phosphoprotein
  • phosvitin
  • polyacrylamide gel electrophoresis (PAGE)
  • protein
  • protein quantitation