An immuno responsive membrane was prepared by immobilizing Wasserman antigen to acetylcellulose. The antigen-binding membrane was used in developing an immunosensor for determining specifically corresponding antibody in serum. The senor is characterized by electrochemical determination of the antigen-antibody complex formed at the membranesoluti on interface. Experimental data on the applicability of the sensor to serology tests for syphilis are given as well as an outline of a possible mechanism for evaluating the electrical potential difference across the antigen-binding membrane.
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Aizawa, M., Suzuki, S., Nagamura, Y. et al. An immunosensor for syphilis. Journal of Solid-Phase Biochemistry 4, 25–31 (1979). https://doi.org/10.1007/BF02991805
- Membrane Potential
- Physiological Saline
- Binding Membrane
- Positive Serum