Springer Nature is making SARS-CoV-2 and COVID-19 research free. View research | View latest news | Sign up for updates

An immunosensor for syphilis


An immuno responsive membrane was prepared by immobilizing Wasserman antigen to acetylcellulose. The antigen-binding membrane was used in developing an immunosensor for determining specifically corresponding antibody in serum. The senor is characterized by electrochemical determination of the antigen-antibody complex formed at the membranesoluti on interface. Experimental data on the applicability of the sensor to serology tests for syphilis are given as well as an outline of a possible mechanism for evaluating the electrical potential difference across the antigen-binding membrane.

This is a preview of subscription content, log in to check access.


  1. 1.

    Guilbault, G. G. (1972) Biotechnol. Bioeng. Symp. 3, p. 361.

  2. 2.

    Clark, Jr.,L. C. (1972) Biotechnol. Bioeng. Symp. 3, p. 377.

  3. 3.

    Gough, D. A., andAndrade, K. E. (1973) Science 180: 380.

  4. 4.

    Rechnits, G. A. (1975) Science 190: 234.

  5. 5.

    Aizawa, M., Karube, I., andSuzuki, S. (1974) Anal. Chim. Acta 69: 431.

  6. 6.

    Aizawa, M., andSuzuki, S. (1976) Denki Kagaku 44: 279.

  7. 7.

    Satoh, I., Karube, I., andSuzuki, S. (1976) Biotechnol. Bioeng. 18: 269.

  8. 8.

    Aizawa, M., Kato, S., andSuzuki, S. (1977) J. Membrane Sci. 2:125.

  9. 9.

    Portnoy, J., Garson, W., andSmith, C. A. (1957) U. S. Public Health Reports 72: 761.

Download references

Author information

Correspondence to Masuo Aizawa or Shuichi Suzuki or Yoichi Nagamura or Rikio Shinohara or Isao Ishiguro.

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Aizawa, M., Suzuki, S., Nagamura, Y. et al. An immunosensor for syphilis. Journal of Solid-Phase Biochemistry 4, 25–31 (1979).

Download citation


  • Membrane Potential
  • Syphilis
  • Physiological Saline
  • Binding Membrane
  • Positive Serum