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Analysis of three-dimensional cell imaging obtained with optical microscopy techniques based on defocusing

Abstract

The properties of an optical microscope are analyzed and analytically evaluated with a simple and effective model in order to understand the true meaning, limitations, and real capabilities of a defocusing technique.

Major emphasis is given to the applications related to microscopic objects of biological interest using fluorescence and absorption light microscopy. A procedure for three-dimensional viewing is analyzed and discussed.

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Correspondence to B. Bianco.

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Bianco, B., Diaspro, A. Analysis of three-dimensional cell imaging obtained with optical microscopy techniques based on defocusing. Cell Biophysics 15, 189–199 (1989). https://doi.org/10.1007/BF02989683

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Index Entries

  • Optical sectioning
  • 3-D cell imaging
  • Fourier optics
  • fluorescence microscopy
  • 3-D point spread function