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Immuno-phenotype and proliferative response of B-cell chronic lymphocytic leukemia

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Abstract

This study examines the immunophenotypic profiles in both pretreated and treated CLL patients which could be useful from the prognosis point of view. Patients suffering from B CLL and having IgG markers were relatively more aggressive than cells bearing IgM phenotypes. Male predominance is observed in male/female ratio in this disease. B CLL showed heterogeneity by showing reactivity against various T cell markers such as CD5 (present on mature T cells) and also CD4 (T helper/inducer), CD8 (T suppressor/cytotoxic) and a very high percentage of 1a (HLA-DR). The proliferative response to cells to stimulation with PHA and PWM indicated that there is a primary defect in the capacity of these small lymphocytes to undergo a proliferative response due to an intrinsic defect in the B lymphocytes. This study also reflects a maturation arrest in the later developmental stage of B lymphopoiesis. The three findings which are novel are the difference in prognosis between IgG-IgM and IgG, the changes in T cell subsets and the mitogenic response.

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Abbreviations

CLL:

Chronic lymphocytic leukemia

B CLL:

B cell type chronic lymphocytic leukemia

CM:

Complete media

MoAbs:

Monoclonal antibodies

FITC:

Fluorescein isothiocyanate

Ig:

Immunoglobulin

PBS:

Phosphate buffered saline

UV:

Ultraviolet

PHA:

Phyto-haemagglutinin

PWM:

Pokeweed mitogen

CPM:

Counts per minute

S.E.:

Standard error

Tγ:

T Suppressor cells

Tμ:

T helper cells

Smlg:

Surface membrane Immunoglobulin

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Correspondence to Sangeeta Karfa or Ramkrishna Gopal or Jagadish S. Nadkarni.

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Karfa, S., Gopal, R. & Nadkarni, J.S. Immuno-phenotype and proliferative response of B-cell chronic lymphocytic leukemia. Med. Oncol. & Tumor Pharmacother. 7, 265–272 (1990). https://doi.org/10.1007/BF02987105

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Key words

  • B CLL
  • Phenotype
  • Lymphocytosis
  • Functional activity