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Comparison of pre-S1 and pre-S2 proteins in hepatocytes with replication status by in situ hybridization assay

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To compare the relationship of pre-S1 and pre-S2 proteins in hepatocytes with HBV replication, HBVDNA in situ hybridization was performed for a group of patients with chronic hepatitis by bio-probe, in conjunction with detection of intrahepatic HBsAg, HBcAg, pre-S1 and-S2 antigens. It was found that the expression of intrahepatic pre-s1 and pre-S2 proteins was visualized as cytoplasmic homogeneous and inclusion types, on some occasions as membranous localization. However, the positive rate for pre-S1 protein was up to 75% (15/20), being significantly higher than that of pre-S2 protein (35%; 7/20) in the same group (P<0.05). What in moreimportant, the intrahepatic pre-S1 expression, rather than pre-s2, was in parallel with the presence of intrahepatic HBVDNA and HBcAg. All these findings suggest that the pre-S1 protein might be mainly expressed at the HBV replication phase, thus, to some extent, the detection of pre-S1 protein might complement HBe system and HBVDNA clinical routine.

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Correspondence to Zhang Yong-yuan.

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Yong-yuan, Z., Zi-qun, Y., You-kun, W. et al. Comparison of pre-S1 and pre-S2 proteins in hepatocytes with replication status by in situ hybridization assay. Journal of Tongji Medical University 10, 150–153 (1990). https://doi.org/10.1007/BF02986453

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Key words

  • pre-S gene
  • pre-S proteins HBV replication
  • HBV markers
  • in as a situ hybridization