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Chinese Journal of Cancer Research

, Volume 13, Issue 3, pp 166–170 | Cite as

The cloning of hrnt-1 using a combination of cdna library screening with biotin-labeled probe and rapid amplification of cDNA ends

  • Zhang Kai-tai Email author
  • Li Gang 
  • Hu Ying-chun 
  • Wu De-chang 
Basic Investigation
  • 31 Downloads

Abstract

Objective: To clone the human counterpart of rat ZA73, EST cloned from rat tracheal epithelial (RTE) neoplastic transformed cell model induced by (a-particles radiation by using mRNA differential display. Methods: According to the sequence of rat ZA73, a probe was biotin-labeled to screen human cDNA library, and then the gene sequence was extended by RACE (rapid amplification of cDNA ends). Result: Human gene HRNT-1 (GenBank Accession Number: AF223393) is 4.256 kb in length, with an ORF located in the region between 254 and 3013 bp. 5′ UTS (untranslated sequences) is 253 bp, 3′ UTS is 1243 bp. Conclusion: The combination of cDNA library screening with biotin-labeled probes and RACE is an effective method to clone full-length cDNA, especially for sequences longer than 2 kb.

Key words

Gene Library screen RACE 

CLC number

Q785 

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Copyright information

© Chinese Journal of Cancer Research 2001

Authors and Affiliations

  • Zhang Kai-tai 
    • 1
    Email author
  • Li Gang 
    • 1
  • Hu Ying-chun 
    • 1
  • Wu De-chang 
    • 1
  1. 1.Department of Molecular ToxicologyBeijing Institute of Radiation MedicineBeijing

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