Skip to main content

Advertisement

SpringerLink
Log in

Establishment of a Monosomy 7 Leukemia Cell Line, MONO-7, With aras Gene Mutation

  • Case Report
  • Published:
International Journal of Hematology Aims and scope Submit manuscript

Abstract

A monosomy 7 leukemia cell line, designated MONO-7, was established from the peripheral blood of a patient with monosomy 7 acute myelocytic leukemia (French-American-British classification M0). The cells were cultured continuously for more than 24 months in RPMI-1640 medium supplemented with 10% heat-inactivated fetal calf serum. The cell line exhibits an unclassified appearance. Cytochemically, α-naphthol-acetate esterase and myeloperoxidase are negative. Immunophenotypi-cally, the cell line expresses CD33, CD13, CD56, CD34, CD38, HLA-DR, and CD45, but lacks T and B cell-associated antigens. Karyotypic analysis of the cell line showed only 45,XY,-7. Analysis of the N-ras gene mutation demonstrated identical mutations in fresh leukemic cells and the MONO-7 cell line. Clonal rearrangements of the immunoglobulin heavy-chain gene, T-cell receptor β-chain gene, or T-cell receptor γ-chain gene were not found in DNA extracted from MONO-7 cells. The growth of MONO-7 cells in vitro was stimulated by recombinant human granulocyte-macrophage colony-stimulating factor or interleukin 3. To our knowledge, this is the first report of the establishment of a cell line with the karyotype 45,XY,-7 with-out any other abnormality and with a ras gene mutation.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Luna-Fineman S, Shannon KM, Lange BJ. Childhood monosomy 7: epidemiology, biology, and mechanistic implications.Blood. 1995;85:1985–1999.

    PubMed  CAS  Google Scholar 

  2. Neubauer A, Shannon K, Liu E. Mutations of the ras proto- oncogenes in childhood monosomy 7.Blood. 1991;77:594–598.

    PubMed  CAS  Google Scholar 

  3. Miyauchi J, Asada M, Sasaki M, Tsunematsu Y, Kojima S, Mizutani S. Mutations of the N-ras gene in juvenile chronic myelogenous leukemia.Blood. 1994;83:2248–2254.

    PubMed  CAS  Google Scholar 

  4. Michiels JJ, Mallios-Zorbala H, Prins ME, Hahlen K, Hagemeijer A. Simple monosomy 7 and myelodysplastic syndrome in thirteen patients without previous cytostatic treatment.Br J Haematol. 1986;64:425–433.

    Article  PubMed  CAS  Google Scholar 

  5. Brandwein JM, Horsman DE, Eaves AC, et al. Childhood myelodysplasia: suggested classification as myelodysplastic syndromes based on laboratory and clinical findings.Am J Pediatr Hematol Oncol. 1990;12:63–70.

    Article  PubMed  CAS  Google Scholar 

  6. Morel P, Hebbar M, Lai JL, et al. Cytogenetic analysis has strong independent prognostic value in de novo myelodysplastic syndromes and can be incorporated in a new scoring system: a report on 408 cases.Leukemia. 1993;7:1315–1323.

    PubMed  CAS  Google Scholar 

  7. Borgstrom G, Teerenhovi L, Vuopio P, et al. Clinical implications of monosomy 7 in acute nonlymphocytic leukemia.Cancer Genet Cytogenet. 1980;2:115–126.

    Article  Google Scholar 

  8. Leverger G, Bernheim A, Daniel MT, Flandrin G, Schaison G, Berger R. Cytogenetic study of 130 childhood acute nonlympho- cytic leukemias.Med Pediatr Oncol. 1988;16:227–232.

    Article  PubMed  CAS  Google Scholar 

  9. Schiffer CA, Lee EJ, Tomiyasu T, Wiernik PH, Testa JR. Prognostic impact of cytogenetic abnormalities in patients with de novo acute nonlymphocytic leukemia.Blood. 1989;73:263–270.

    PubMed  CAS  Google Scholar 

  10. de Planque MM, Kluin-Nelemans HC, van Krieken HJ, et al. Evolution of acquired severe aplastic anaemia to myelodysplasia and subsequent leukaemia in adults.Br J Haematol. 1988;70:55–62.

    Article  PubMed  CAS  Google Scholar 

  11. Imashuku S, Hibi S, Nakajima F, et al. A review of 125 cases to determine the risk of myelodysplasia and leukemia in pediatric neutropenic patients after treatment with recombinant human granulocyte colony-stimulating factor.Blood. 1994;84:2380–2381.

    PubMed  CAS  Google Scholar 

  12. Johansson B, Mertens F, Heim S, Kristoffersson U, Mitelman F. Cytogenetics of secondary myelodysplasia (sMDS) and acute non- lymphocytic leukemia (sANLL).Eur J Haematol. 1991;47:17–27.

    Article  PubMed  CAS  Google Scholar 

  13. Le Beau MM, Albain KS, Larson RA, et al. Clinical and cytoge- netic correlations in 63 patients with therapy-related myelodys- plastic syndromes and acute nonlymphocytic leukemia: further evidence for characteristic abnormalities of chromosomes no. 5 and 7.J Clin Oncol. 1986;4:325–345.

    Article  PubMed  CAS  Google Scholar 

  14. Whang-Peng J, Young RC, Lee EC, Longo DL, Schechter GP, DeVita VT Jr. Cytogenetic studies in patients with secondary leukemia/dysmyelopoietic syndrome after different treatment modalities.Blood. 1988;71:403–414.

    PubMed  CAS  Google Scholar 

  15. Cuneo A, Fagioli F, Pazzi I, et al. Morphologic, immunologic and cytogenetic studies in acute myeloid leukemia following occupational exposure to pesticides and organic solvents.Leuk Res. 1992;16:789–796.

    Article  PubMed  CAS  Google Scholar 

  16. Golomb HM, Alimena G, Rowley JD, Vardiman JW, Testa JR, Sovik C. Correlation of occupation and karyotype in adults with acute nonlymphocytic leukemia.Blood. 1982;60:404–411.

    CAS  PubMed  Google Scholar 

  17. Mitelman F, Nilsson PG, Brandt L, Alimena G, Gastaldi R, Dallapiccola B. Chromosome pattern, occupation, and clinical features in patients with acute nonlymphocytic leukemia.Cancer Genet Cytogenet. 1981;4:197–214.

    Article  CAS  PubMed  Google Scholar 

  18. Paietta E, Gucalp R, Wiernik PH. Monosomy 7 in multilineage and acute lymphoblastic leukaemia.Br J Haematol. 1991;79:152–155.

    Article  PubMed  CAS  Google Scholar 

  19. Lau RC, Squire J, Brisson L, et al. Lymphoid blast crisis of B-line- age phenotype with monosomy 7 in a patient with juvenile chronic myelogenous leukemia (JCML).Leukemia. 1994;8:903–908.

    CAS  PubMed  Google Scholar 

  20. Hogge DE, Shannon KM, Kalousek DK, et al. Juvenile monosomy 7 syndrome: evidence that the disease originates in a pluripotent hemopoietic stem cell.Leuk Res. 1987;11:705–709.

    Article  PubMed  CAS  Google Scholar 

  21. Sieff CA, Chessells JM, Harvey BA, Pickthall VJ, Lawler SD. Monosomy 7 in childhood: a myeloproliferative disorder.Br J Haematol. 1981;49:235–249.

    Article  PubMed  CAS  Google Scholar 

  22. Neuman WL, Rubin CM, Rios RB, et al. Chromosomal loss and deletion are the most common mechanisms for loss of heterozy- gosity from chromosomes 5 and 7 in malignant myeloid disorders.Blood. 1992;79:1501–1510.

    PubMed  CAS  Google Scholar 

  23. Gerritsen WR, Donohue J, Bauman J, et al. Clonal analysis of mye- lodysplastic syndrome: monosomy 7 is expressed in the myeloid lineage, but not in the lymphoid lineage as detected by fluorescent in situ hybridization.Blood. 1992;80:217–224.

    PubMed  CAS  Google Scholar 

  24. Chan LC, Sheer D, Drysdale HC, Bevan D, Greaves MF. Mono- somy 7 and multipotential stem cell transformation.Br J Haematol. 1985;61:531–539.

    Article  PubMed  CAS  Google Scholar 

  25. Sanz GF, Gomis F, Sanz MA. Monosomy 7 and acute mixed leukemia.Br J Haematol. 1986;64:842–844.

    Article  PubMed  CAS  Google Scholar 

  26. Fujisaki H, Hara J, Takai K, et al. Lineage switch in childhood leukemia with monosomy 7 and reverse of lineage switch in severe combined immunodeficient mice.Exp Hematol. 1999;27:826–833.

    Article  PubMed  CAS  Google Scholar 

  27. An International System for Human Cytogenetic Nomenclature (1985). ISCN 1985. Report of the Standing Committee on Human Cytogenetic Nomenclature.Birth Defects Orig Artic Ser. 1985;21:1–117.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Developmental Medicine (Pediatrics), D-5, Osaka University Graduate School of Medicine, Osaka, Japan

    Hiroyuki Fujisaki, Kenji Takai, Akihisa Sawada, Sadao Tokimasa, Yoshiko Matsuda, Hideaki Ohta, Yuko Osugi, Ji Yoo Kim & Junichi Hara

  2. Department of Pediatrics, Osaka City General Hospital, Osaka, Japan

    Gaku Hosoi & Masahiro Sako

Authors
  1. Hiroyuki Fujisaki
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Kenji Takai
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Akihisa Sawada
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Sadao Tokimasa
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Yoshiko Matsuda
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Hideaki Ohta
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Yuko Osugi
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Ji Yoo Kim
    View author publications

    You can also search for this author in PubMed Google Scholar

  9. Gaku Hosoi
    View author publications

    You can also search for this author in PubMed Google Scholar

  10. Masahiro Sako
    View author publications

    You can also search for this author in PubMed Google Scholar

  11. Junichi Hara
    View author publications

    You can also search for this author in PubMed Google Scholar

About this article

Cite this article

Fujisaki, H., Takai, K., Sawada, A. et al. Establishment of a Monosomy 7 Leukemia Cell Line, MONO-7, With aras Gene Mutation. Int J Hematol 75, 72–77 (2002). https://doi.org/10.1007/BF02981983

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02981983

Key words

Search

Navigation