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Synergistic interactions between phenylamide fungicides and respiration inhibitors in controlling phenylamide-resistant populations ofPhytophthora infestans on potato tuber disks

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Abstract

The efficacy of mixtures of respiration inhibitors and phenylamide fungicides (oxadixyl and metalaxyl) in controlling late blight was investigated using potato tuber disks. Results showed that uncoupling agents(e.g. DNP), inhibitors of ATP formation(e.g. oligomycin), ionophores(e.g, valinomycin) and inhibitors that block specific carriers in the electron transport chain(e.g. rotenone, antimycin A) increased significantly the control efficacy of both metalaxyl and oxadixyl towards populations ofPhytophthora infestons when applied to tuber slices pretreated with sublethal doses of both fungicides. When applied alone at the same doses, respiration inhibitors did not inhibit the development of the late blight fungus. Increased control efficacy of mixtures was not noted against phenylamide-sensitive populations of the fungus.

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References

  1. 1.

    Bashan, B. (1989) [Inhibition of oomyceteous fungal pathogens by systemic fungicides: cellular and metabolic aspects.] Ph.D. thesis, Bar-Ilan University, Ramat Gan, Israel (in Hebrew).

  2. 2.

    Bastian, B, Kadish, D., Levy, Y. and Cohen, Y. (1989) Infectivity to potato, sporangial germination, and respiration of isolates ofPhytophthora infestons from metalaxyl-sensitive and metalaxyl- resistant populations.Phytopathology 79:832–836.

  3. 3.

    Brenneman, J.A. and Black, L.L. (1979) Respiration and terminal oxidases in tomato leaves infected byPhytophthora infestons.Physiol. Plant Pathol. 14:281–290.

  4. 4.

    Gisi, U., Binder, H. and Rimbach, E. (1985) Synergistic interactions of fungicides with different modes of action.Trans. Br. Mycol. Soc. 85:299–306.

  5. 5.

    Gisi, U., Han, J., Sandmeier, R. and Wiedmer, H. (1983) A new systemic oxazolidinone fungicide (SAN 371 F) against diseases caused by peronosporales.Meded. Vac. Landbouwwet. Rijksuniv. Gent. 48:541–549.

  6. 6.

    Kaars Sijpesteijn, A. (1982) Mechanism of action of fungicides,in: Dekker, J. and Georgopoulos, S.G. [Eds.] Fungicide Resistance in Crop Protection, pp. 32–45. PUDOC, Wageningen, The Netherlands.

  7. 7.

    Kaars Sijpesteijn, A. and van der Kerk, G.J.M. (1954) Investigation on organic fungicides. The biochemical mode of action of bisdithiocarbamates and diisothiocyanates. Biochim.Biophys. Acta 13:545–552.

  8. 8.

    Kable, P.F. and Jeffery, H. (1980) Selection for tolerance in organisms exposed to sprays of biocide mixtures: A theoretical model.Phytopathology 70:8–12.

  9. 9.

    Levy, Y., Benderly, M. and Cohen, Y. (1991) Disease development and buildup of resistance to oxadixyl in potato crops inoculated withPhytophlhora infestons as affected by oxadixyl and oxadixyl mixtures: experimental and simulation studies.J. Phytopalhol. 127: (in press).

  10. 10.

    Levy, Y., Benderly, M., Cohen, Y., Gisi, U. and Bassand, D. (1986) The joint action of fungicides in mixtures: Comparison of two methods for synergy calculation.EPPO Bull. 16:651–657.

  11. 11.

    Levy, Y., Levi, R. and Cohen, Y. (1983) Buildup of a pathogen subpopulation resistant to a systemic fungicide under various control strategies: A flexible simulation model.Phytopathology 73: 1475–1480.

  12. 12.

    Levy, Y. and Strizyk, S. (1985) Essai de représentation et d’évaluation des fonctions interaction entre un mélange de fongicides d’une part et d’unités d’infection d’autre part. Publication of ACTA, Paris.

  13. 13.

    Pathak, N. and Clarke, D.D. (1987) Studies on the resistance of the outer cortical tissues of the tubers of some potato cultivars toPhytophthora infestons.Physiol. Mol. Plant Pathol. 31:123–132.

  14. 14.

    Samoucha, Y. and Cohen, Y. (1986) Efficacy of systemic and contact fungicide mixtures in controlling late blight in potatoes.Phytopathology 76:855–8.59.

  15. 15.

    Samoucha, Y. and Cohen, Y. (1988) Synergistic interactions of cymoxanil mixtures in the control of metalaxyl-resistantPhylophthoora infestons of potato.Phytopathology 78:636–340.

  16. 16.

    Samoucha, Y. and Gisi, U. (1987) Possible explanations of synergism in fungicide mixtures againstPhytophthora infestons.Ann. Appl. Biol. 110:303–311.

  17. 17.

    Samoucha, Y., Levy, R.S. and Cohen, Y. (1988) Efficacy over time of cymoxanil mixtures in controlling late blight in potatoes incited by a phenylamide-resistant isolate ofPhytophthora infestons.Crop Prot. 7:210–215.

  18. 18.

    Sanders, P.L., Houser, W.J., Parish, P.J. and Cole, H. Jr. (1985) Reduced-rate fungicide mixtures to delay fungicide resistance and to control selected turfgrass diseases.Plant Dis. 69:939–943.

  19. 19.

    Scardavi, A. (1966) Synergism among fungicides.Annu. Rev. Phylopathol. 4:335–348.

  20. 20.

    Scheepens, P.C. and Fehrmann, H. (1978) Mitochondrial electron transport inPeronosporales I. Isolation and properties ofPythium debaryanum andPhytophthora infestons mitochondria.Phytopalhol. Z. 92: 217–230.

  21. 21.

    Skylakakis, G. (1981) Effects of alternating and mixing pestides on the buildup of fungal resistance.Phytopathology 71: 1119–1121.

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Correspondence to Bilha Bashan or Y. Levy or Y. Cohen.

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Bashan, B., Levy, Y. & Cohen, Y. Synergistic interactions between phenylamide fungicides and respiration inhibitors in controlling phenylamide-resistant populations ofPhytophthora infestans on potato tuber disks. Phytoparasitica 19, 73 (1991). https://doi.org/10.1007/BF02981013

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Key Words

  • Phenylamides
  • respiration inhibitors
  • Phytophthora infestans