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In vitro Variability of a number of characteristics ofSclerotinia sclerotiorum

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Studies were made of the variability ofSclerotinia sclerotiorum (Lib.) de Bary: (i) growth rate when cultivated on potato-dextrose agar (PDA); (ii) thein vitro resistance of this organism to the fungicides benomyl, vinclozolin and procymidone; and (iii) the size and number of sclerotia formed on PDA. The isolates used were collected from eggplants, French beans and tomatoes cultivated in the same locality, but although marked differences were observed among them, especially in growth rate and formation of sclerotia, it was not possible to establish groups or categories among the isolates studied, or to relate the observed variability to their origins. Temperature was a decisive factor for formation of sclerotia on PDA. No correlation was found between size and number of sclerotia, and growth rate. The ED50 level for both vinclozolin and procymidonein vitro was 2 mg l−1, whereas that for benomyl was below the minimum level used, and therefore could not be established.

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Correspondence to J. A. Torés.

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Torés, J.A. In vitro Variability of a number of characteristics ofSclerotinia sclerotiorum . Phytoparasitica 18, 321–329 (1990).

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Key Words

  • Sclerotinia sclerotiorum
  • in vitro variability
  • mycelial growth
  • fungicide effectiveness
  • number and size of sclerotia