A stereoselective and sensitive high performance liquid chromatography using fluorescence detector was examined for the determination of R(−) and S(+)-salbutamol in human plasma. Solid phase extraction method using silica as sorbent was used to extract salbutamol racemates from the plasma matrices. After fractionation and freeze-drying of the eluates containing salbutamol racemates, they were separated and quantified on a chiral stationary column. The detection limit of each enantiomer was 2 ng/ml in human plasma (S/N=3).
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Ariens, E. J., Stereochemistry. a basis for sophisticated nonsense in pharmacokinetics and clinical pharmacology.Eur. J. Clin. Pharmacol., 26, 663–668 (1984).
Bland, R. E., Tanner, R. J. N., Chem, W. H., Lang, J. R., and Powel, J. R., Determination of albuterol in human plasma using solid-phase extraction and high performance liquid chromatography with fluorescence detection.J. Pharm. Biomed. Anal., 8, 591–596 (1990).
Borgstom, L., Nyberg, L., Gonsson, S., Lindberg, C., and Paulson, J., Pharmacokinetic evaluation in man of terbutaline given as separate enantiomers and as racemate.British Journal of Clinical Pharmacology, 27, 49–56 (1989).
Chin, S. K., Hui, A. C., and Giacomini, K. M., High performance chromatographic dertermination of the enantiomer of β-adrenoceptor blocking agents in biological fluid.J. Chromatogr., 489, 438–445 (1989).
Emn, T., and Perkal, M. B., Determination of albuterol in human serum by RP-HPLC with electrochemical detection.J. Chromatogr., 427, 188–194 (1988).
Mehvar, R., Liquid chromatographic analysis of atenolol enantiomer in human plasma and urine.J. Pharm. Sci., 78, 1035–1039 (1989).
Morgan, D. J., Clinical pharmacokinetics of β-agonists.Clin. Pharmacokinet, 18, 270–294 (1990).
Powell, W. L., Weisberger, M., Gural, R., Chung, M., Patrik, J. E., Radwanski, E., and Symchowise, S. S., Camparative bioavailability and pharmacokinetics of three formulation of Albuterol.J. Pharm. Sci., 74, 217–219 (1985).
Tan, Y. K., and Soldin, S. J., Analysis of salbutamol enantiomers in human urine by chiral high performance liquid chromatography and preliminary studies related to the stereoselective disposition kinetics in man.J. chromatogr., 422, 187–195 (1989).
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Seo, J.M., Kim, K.H. Chiral separation of salbutamol enantiomers in human plasma. Arch. Pharm. Res. 17, 244–248 (1994). https://doi.org/10.1007/BF02980455
- Solid phase extraction