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Chiral separation of salbutamol enantiomers in human plasma

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A stereoselective and sensitive high performance liquid chromatography using fluorescence detector was examined for the determination of R(−) and S(+)-salbutamol in human plasma. Solid phase extraction method using silica as sorbent was used to extract salbutamol racemates from the plasma matrices. After fractionation and freeze-drying of the eluates containing salbutamol racemates, they were separated and quantified on a chiral stationary column. The detection limit of each enantiomer was 2 ng/ml in human plasma (S/N=3).

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Correspondence to Kyeong Ho Kim.

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Seo, J.M., Kim, K.H. Chiral separation of salbutamol enantiomers in human plasma. Arch. Pharm. Res. 17, 244–248 (1994). https://doi.org/10.1007/BF02980455

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Key words

  • Enantiomer
  • Salbutamol
  • Solid phase extraction