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Replication, molecular biology, and genetic engineering of granulosis viruses

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Baculoviruses of subgroup B, the granulosis viruses, show particular promise as control agents for several horticultural pests. Recent studies have concentrated particularly onCydia pomonella granulosis virus, which provides very good control of the codling moth, a major world-wide pest of apples, pears and walnuts. The viral genome has been mapped and the granulin gene has been located and sequenced. Transfer vectors for insertion of foreign genes into the virus have been constructed. Cell cultures which support viral replication have been obtained and have been transfected with viral DNA.

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  1. Akiyoshi, D.; Chakerian, R.; Rohrmann, G.F.; Nesson, M.H.; Beaudreau, G.S. (1985) Cloning and sequencing of the granulin gene fromTrichoplusia ni granulosis virus.Virology 141, 328–332.

  2. Blissard, G.W.; Rohrmann, G.F. (1990) Baculovirus diversity and molecular biology.Annual Review of Entomology 35,127–155.

  3. Chakerian, R.; Rohrmann, G.F.; Nesson, M.H.; Leisy, D.J.; Beaudreau, G.S. (1985) The nucleotide sequence of thePieris brassicae granulosis virus granulin gene.Journal of General Virology 66,1263–1269.

  4. Crook, N.E. (1991) Baculoviridae: Subgroup B. Comparative aspects of granulosis viruses. In: Kurstak, E. [Ed.] Viruses of Invertebrates. Marcel Dekker, New York, NY. pp. 73–110.

  5. Crook, N.E.; Spencer, R.A.; Payne, C.C.; Leisy, D.J. (1985) Variation inCydia pomonella granulosis virus isolates and physical maps of the DNA from three variants.Journal of General Virology 66, 2423–2430.

  6. Dwyer, K.G.; Granados, R.R. (1987) A physical map of thePieris rapae granulosis virus genome.Journal of General Virology 68,1471–1476.

  7. Rohrmann, G.F. (1986) Polyhedrin structure.Journal of General Virology 67, 1499–1513.

  8. Smith, G.E.; Summers, M.D. (1982) DNA homology among subgroup A, B and C baculoviruses.Virology 123, 393–406.

  9. Smith, I.R.L. (1987) Genome Structure and Host Interactions ofPieris granulosis virus. Ph.D. Thesis, University of London, UK.

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Correspondence to N. E. Crook or D. Winstanley.

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Crook, N.E., Winstanley, D. Replication, molecular biology, and genetic engineering of granulosis viruses. Phytoparasitica 20, S33–S36 (1992).

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  • Recombinant Virus
  • Transfer Vector
  • Nuclear Polyhedrosis Virus
  • Amino Acid Homology
  • Nucleotide Homology