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Further suppression ofBotrytis cinerea disease in cucumber seedlings by chitosan-copper complex as compared with chitosan alone

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Chitosan-copper complex compared with chitosan alone enhanced suppression ofBotrytis cinerea rot development on four-true-leaf cucumber seedlings in controlled growth chambers. This paper constitutes the first report of such enhancement. The optimal concentrations for the most effective suppression ofBotrytis development were 0.2 gl −1 chitosan and 1.6 mmole copper. After 12 days’ incubation, marked and significantly better disease suppression was obtained with chitosan-copper complex (75% suppression) than with chitosan alone. The chitosan-copper complex could be a very promising decay control agent for use in both conventional and organic agriculture.

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  1. 1.

    Benhamou, N. and Theriault, G. (1992) Treatment with chitosan enhances resistance of tomato plants to the crown and root rot pathogenFusarium oxysporum f.sp.radicis-lycopersici.Physiol. Mol. Plant Pathol. 41:33–52.

  2. 2.

    Ben-Shalom, N., Ardi, R., Aki, C., Kudabeava, N. and Fallik, E. (2003) Controlling gray mould caused byBotrytis cinerea in cucumber plants by means of chitosan.Crop Prot. 22(2):in press.

  3. 3.

    Braier, N.C. and Jishi, R.A. (2000) Density functional studies of Cu2+ and Ni2+ binding to chitosan.J. Mol. Struct. 499:51–55.

  4. 4.

    Elad, Y. (1997) Responses of plants to infection byBotrytis cinerea and novel means involved in reducing their susceptibility to infection.Biol. Rev. 72:381–422.

  5. 5.

    El Ghaouth, A., Arul, J., Benhamou, N., Asselin, A. and Belanger, R.R. (1994) Effect of chitosan on cucumber plants: suppression ofPythium aphanidermatum and induction of defense reactions.Phytopathology 84:313–320.

  6. 6.

    El Ghaouth, A., Arul, J., Wilson, C. and Benhamou, N. (1997) Biochemical and cytochemical aspects of the interactions of chitosan andBotrytis cinerea in bell pepper fruit.Postharv. Biol. Technol. 12:183–194.

  7. 7.

    Hadwiger, L.A., Chiang, C., Victory, S. and Horovitz, D. (1988) The molecular biology of chitosan in plant-pathogen interaction and its application to Skjak, G., Anthonsen, B.T. and Stanford, P. [Eds.] Chitin and Chitosan: Sources, Chemistry, Biochemistry, Physical Properties and Applications. Elsevier Applied Sciences, Amsterdam, the Netherlands. pp. 119–138.

  8. 8.

    Jacobsen, B.J. and Backman, A. (1993) Biological and cultural plant disease controls: alternatives and supplements to chemicals, in IPM systems.Plant Dis. 77:311–315.

  9. 9.

    Roberts, G.A.F. (1992) Complexes formation with metals Chitin Chemistry. Macmillan, London, UK. pp. 206–216.

  10. 10.

    Stirling, A.M., Pegg, K.G., Hayward, A.C. and Stirling, G.R. (1999) Effect of copper fungicide onColletortrichum gloeosporioides and other microorganisms on avocado leaves and fruit.Aust. J. Agric. Res. 50:1459–1468.

  11. 11.

    Tiuterev, S., Yakubchik, M., Tarlakovsky, S., Popova, E., Vysky, V. and Dorofeyeva, T. (1996) Chitosan: mechanism of action and ways of using as ecologically safe means in enhancement of plant disease resistance.Arch. Phytopathol. Pflanzenschutz 30:323–332.

  12. 12.

    Young, D.D. and Kauss, H. (1983) Release of calcium from suspension-culturedGlycine max cells by chitosan, other polycations, and polyamines in relation to effects of membrane permeability.Plant Physiol. 73:698–702.

  13. 13.

    Yourman, L.F. and Jeffers, S.N. (1999) Resistance to benzimidazole and dicarboximide fungicides in greenhouse isolates ofBotrytis cinerea.Plant Dis. 83:569–575.

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Correspondence to E. Fallik.

Additional information posting Dec. 18, 2002.

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Ben-Shalom, N., Fallik, E. Further suppression ofBotrytis cinerea disease in cucumber seedlings by chitosan-copper complex as compared with chitosan alone. Phytoparasitica 31, 99 (2003).

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Key words

  • Cucumis sativus
  • gray mold
  • Botrytis cinerea suppression
  • chitosan
  • copper