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Flavonoids from the flower ofRhododendron yedoense var.Poukhanense and their antioxidant activities

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Abstract

Antioxidant flavonoids have been isolated from the flower ofRhododendron yedoense var.poukhanense. One new flavonoid and three known flavonoids, quercetin-5-O-β-D-glucopyranoside (1), quercetin (3), and quercitrin (4), were isolated from the butanol and ethyl acetate extracts of the plant. The new flavonoid was identified as myricitrin-5-methyl ether (2). The isolation of these flavonoids from this plant, for the first time, is a valuable finding. The flavonoids were evaluated for their antioxidant activities using 1,1-diphenyl-2-picrylhydrazyl free radical (DPPH), TBARS (thiobarbituric acid reactive substance) and Superoxide anion radical (O2) in the xanthine/xanthine oxidase assay system. In the DPPH scavenging assay, the IC50 values were 4.5±0.48 u.M for compound 2 and 9.7±0.29 μ.M for compound 3, which showed an antioxidant activity approximately 1.5-2 times higher than the antioxidant activity of α-tocopherol (9.8±0.94 μM). Additionally, the antioxidant activities of myricitrin-5-methyl ether (2) (IC50 = 1.7±0.22 μM) and quercetrin (4) (IC50 = 1.9±0.63 μM) were higher than that of L-ascorbic acid (IC50= 7.4±0.63 μM) when evaluated using a TBARS assay. Compound 2 showed high activity in both the inhibition of xanthine oxidase (1.1±0.21 mM) and in the activation of Superoxide scavenging.

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Correspondence to Sung Je Jung or Dong-Hyun Kim or Yoon-Hee Hong or Jin-Hee Lee or Hyo-Nam Song or Young-Deok Rho or Nam-In Baek.

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Jung, S.J., Kim, D., Hong, Y. et al. Flavonoids from the flower ofRhododendron yedoense var.Poukhanense and their antioxidant activities. Arch Pharm Res 30, 146–150 (2007). https://doi.org/10.1007/BF02977686

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Key words

  • Rhododendron yedoense
  • Ericaceae
  • Antioxidant activity
  • DPPH scavenging assay
  • TBARS assay
  • Xanthine oxidase inhibition assay
  • Superoxide anion radical scavenging assay
  • Myricitrin-5-methyl ether