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Determination of aloesin in plasma by high-performance liquid chromatography as fluorescent 9-anthroyl derivative

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A sensitive high-performance liquid chromatographic (HPLC) method for the determination of aloesin in plasma was developed. After solid-phase extraction from plasma and derivatization of aloesin and compound AD-1, which was prepared from aloesin as a internal standard, with 9-anthroylnitrile in the presence of quinuclidine, the derivatives were separated on a Inertsil ODS-3 column using acetonitrile/methanol/water (3∶1∶6) as a mobile phase, and detected fluorimetrically at 460 nm with excitation at 360 nm. The detection limit of aloesin was 3.2 ng/ml in plasma (S/N=3).

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Correspondence to Kyeong Ho Kim.

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Kim, K.H., Lee, J.G., Park, J.H. et al. Determination of aloesin in plasma by high-performance liquid chromatography as fluorescent 9-anthroyl derivative. Arch. Pharm. Res. 21, 651 (1998).

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Key words

  • Aloesin
  • 9-Anthroylnitrile
  • Fluorescent labelling reagent
  • Reversed-Phase HPLC