The antitumor activity ofBifidobacterium breve K-110, and K-111, andB. infantis K-525 was investigated. TheseBifidobacterial cells and their cell wall preparations (WPG) significantly increased the survival rate of mice who had been intraperitoneally implanted with sarcoma 180 cells. Solid tumor growth was inhibited even when the sarcoma 180 cells were implanted into the groins of the mice. However, theBifidobacterial cells did not showin vitro cytotoxicity against tumor cell lines. Cell kinetic studies revealed that these WPGs induced neutrophils, which were followed by macrophages, at the site of peritoneal injection. The WPGs directly activated these cells to inhibit the growth of tumor cells inin vitro assays. Our results suggest thatBifidobacterial WPGs induced and activate nonspecific phagocytes,in situ to reject growing tumor cells in the mouse peritoneal cavity.
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Adachi, S., Lactic acid bacteria, and the control of tumors, In B. J. B. Wood (Eds.)The Lactic acid bacteria, vol. 1, Elsevier Science Publishers, Ltd. Essex, UK, pp. 233–261, 1992.
Carmichael, J., DeGreff, W. G., Gazdar, A. F., Minna, J. D. and Mitchell J. B., Evaluation of tetrazolium-based semiautomated colorimetric assay: assessment of chemosensitivity testing.Cancer Res., 47, 936–940 (1987).
Clemmesen, J., antitumor effect of lactobacilli substances:L. bulgaricus effect.Mol. Biother., 1, 279–282 (1989).
Fukui, H. and Reynolds, W., Antitumor activity of a Streptococcus pyogenes preparation (OK-432). I. Sequential effector mechanisms following a single OK-432 injection in F344 rats leading to the rejection of syngeneic MADB 106 tumor cells.J. Natl. Cancer Inst., 79, 1011–1017 (1987).
Han, M. J., Park, H.-Y., and Kim, D.-H., Protective effects ofBifidobacterium spp. on experimental colon carcinogenesis with 1,2-dimethylhydrazine.J. Microbiol. Biotechnol., 9, 368–370 (1999).
Hashimoto, Y., Kowwi, Y. and Tamura, Z., Antitumor effect of Bifidobacterium and a streptococcal preparation in experimental tumor systems. In W.D. Terry, and Y. Yamamura (Eds.),Immunology and immunotherapy of cancer, Elsevier, North-Holland. pp. 351–360, 1979.
Kao, I., Kobayashi, S., Yokokura, T. and Mutai, M., Antitumor activity ofLactobacillus casei in mice.Gann., 72, 517–523 (1981).
Keller, R., Keist, R., and Schwendener, R. A., Discrepancy in the abilities of lymphokines and bacteria to mediate tumor protectionin vivo and/or tumoricidal activity by macrophagesin vitro Int. J. Cancer, 44, 512–517 (1989).
Nikonenko, B. V., Apt, A. S., Mezhlumova, M. B., Avidenko, V. G., Yerenev, V. V., and Moroz, A. M., Influence of the mouse Bcq, Tbc-1 and xid genes on resistance and immune responses to tuberculosis infection and efficacy ofBacille Calmette-Guerin (BCG) vaccination.Clin. Exp. Immunol., 104, 37 (1996).
Park, H.-Y., Bae, E.-A., Han, M. J., Choi, E.-C. and Kim, D.-H., Inhibitory effects of Bifidobacteirum spp. isolated from a healthy Korean on harmful enzymes of human intestinal microflora.Arch. Pharm. Res., 21, 54–61 (1998).
Salminen, S., Deighton, and Gorbach, S., Lactic acid bacteria in health and disease, In Salminen, S. and von Wright, A. (Eds.),Lactic acid bacteria, Marcel Dekker, Inc., New York, pp. 199–226, 1974.
Sekine, K., Toida, T., Saito, M., Kuboyama, M., Kawashima, T. and Hashimoto, Y., A new morphologically characterized cell wall preparation (whole peptidogycan) fromBifidobacterium infantis with a higher efficacy on the regression on an established tumor in mice.Cancer Res., 45, 1300–1307 (1985).
Sekine, K., Ohta, J., Tatsuki T., Ohnishi, M., Toida, T., and Kawashima, T., Significance of bacterial structure of WPG related to antitumoral activities.Biotherapy, 6, 329–330 (1990).
Sekine, K., Watanabe-Sekine, E., Ohta, J., Toida, T. Tatsuki, T., Kawashima, T. and Hashimoto Y., Induction and activation of tumoricidal cellsin vivo andin vitro by the bacterial cell wall ofBifidobacterium infantis.Bifidobacteria Bifidobacteria, 13, 65–77 (1994).
Sekine, K., Ohta, J., Onishi, M., Tatsuki, T., Shimokawa, Y., Toida, T., Kawashima, T. and Hashimoto, Y., Analysis of antitumor properties of effector cells stimulated with a cell wall preparation (WPG) ofBifidobacterium infantis.Biol. Pharm. Bull., 18, 148–153 (1995).
Simon, G. L. and Gorbach, S. L., Intestinal flora in health and disease.Gastroenterol., 86, 174–193 (1984).
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Rhee, Y.-., Bae, E.-., Kim, S.-. et al. Antitumor activity ofBifidobacterium spp. isolated from a healthy Korean. Arch Pharm Res 23, 482–487 (2000). https://doi.org/10.1007/BF02976577
- Antitumor activity
- Cell wall