The antioxidant activity ofArtemisia montana was determined by measuring the radical scavenging effect on 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical and inhibitory activity against free radical generation of hepatocytes (AC2F). The methanol extract ofA. montana showed strong radical scavenging activity at a concentration of 10.1 μg/ml, and thus fractionated by solvent extraction. Esculetin and luteolin 7-O-rutinoside (scolymoside) were isolated as the active principles from the EtOAc and Interphase fractions, respectively. The antioxidant activity of these compounds were comparable to that of L-ascorbic acid.
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Blois, M. S., Antioxidant determinations by the use of a stable free radical.Nature, 26, 1199–1200 (1958).
Choi, J. S., Lee, J. H., Park, H. J., Kim, H. G., Young, H. S. and Mun, S. I., Screening for antioxidant activity of plants and marine algae and its active principles fromPrunus davidiana.Kor. J. Pharmacogn., 24(4), 299–303 (1993).
Choi, J. S. and Woo, W. S., Coumarins and triterpenoid glycosides from the roots ofPatrinia scabiosaefolia.Arch. Pharm. Res., 7(2), 121–126 (1984).
Cuvelier, M-E., Richard, H. and Berset, C., Comparison of the antioxidative activity of some acid-phenols: Structure-activity relationship.Biosci. Biotech. Biochem., 56(2), 324–325 (1992).
Hirai, Y., Sanada, S., Ida, Y. and Shoji, J., Studies on the constituents of Palmae plants. I. The constituents ofTrachycarpus fortunei (HOOK). H. WENDL. (1)Chem. Pharm. Bull., 32(1), 295–301 (1984).
Hoult, J. R. S. and Paya, M., Pharmacological and biochemical actions of simple coumarins: Natural products with therapeutic potential.Gen. Pharmac., 27(4), 713–722 (1996).
Kim, S. S., Lee, C. K., Kang, S. S., Jung, H. A. and Choi, J. S., Chlorogenic acid, an antioxidant principle from the aerial parts ofArtemisia iwayomogi that acts on 1,1-diphenyl-2-picrylhydrazyl radical.Arch. Pharm. Res., 20(2), 148–154 (1997).
Kim, T. J.,Korean Resources Plants (1st Ed.), Seoul National University Press, Korea, pp. 265 (1996).
Koreeda, M., Nagaki, M., Hayami, K. I. and Matsueda, S., Studies on sesquiterpene lactones. XIII. Chemical constituent ofArtemisia montana (Nakai) Pamp. Yakugaku Zasshi, 108(5), 434–436 (1988).
Label, C. P. and Bondy, S. C., Sensitive and rapid quantitation of oxygen reactive species formation in rat synaptosomes.Neurochem. Int., 17, 435–441 (1990).
Larson, R. A., The antioxidants of higher plants.Phytochemistry, 27, 969–978 (1998).
Matsueda, S. and Nagaki, M., Studies on sesquiterpenelactones. XII. Chemical constituent ofArtemisia montana (Nakai) Pamp. Yakugaku Zasshi, 104(7), 753–756 (1984).
Nagaki, M. and Matsueda, S., Guaianolides fromArtemisia montana.Phytochemistry, 28, 2731–2733 (1989).
Okuda, T., Hatano, T., Agata, I., Nishibe, S. and Kimura, K., Tannins inArtemisia montana, A. princeps and related species of plant.Yakugaku Zasshi, 106(10), 894–899 (1986).
Ruiz-Larrea, M. B., Leal, A. M., Liza, M., Lacort, M and de Groot, H., de Groot, Antioxidant effect of estradiol and 2-hydroxyestradiol on iron-induced lipid peroxidation of rat liver microsomes.Steroids, 59, 383–388 (1994).
Yoshida, T., Mori, K., Hatano, T., Okumura, T., Uehara, I., Komagoe, K., Fujita, Y. and Okuda, T., Studies on inhibition mechanism of antioxidation by tannins and flavonoids. V. Radical-scavenging effects of tannins and related polyphenols on 1,1-diphenyl-2-picrylhydrazyl radical.Chem. Pharm. Bull., 37, 1919–1921 (1989) and references cited therein.
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Kim, N.M., Kim, J., Chung, H.Y. et al. Isolation of luteolin 7-O-rutinoside and esculetin with potential antioxidant activity from the aerial parts ofArtemisia montana . Arch Pharm Res 23, 237–239 (2000). https://doi.org/10.1007/BF02976451
- Artemisia montana
- Luteolin 7-O-rutinoside
- Antioxidant activity