Springer Nature is making SARS-CoV-2 and COVID-19 research free. View research | View latest news | Sign up for updates

Effect of ginseng saponin on gap junction channel reconstituted with connexin32

  • 30 Accesses

  • 5 Citations

Abstract

Panax-ginseng saponin has been known to exert various pharmacological effects on cellular metabolism. This study was performed to determine the effect of ginseng saponin on gap junction channel-mediated intercellular communication, using an establishedin vitro system of reconstituted gap junction channels. Gap junction channels are a specialized plasma membrane fraction, which are permeable to relatively large water-soluble molecules. The sucrose permeable property of reconstituted gap junction channels was completely inhibited with 0.1% (w/v) of ginseng saponin. We also compared the effect of ginseng saponin with that of Triton X-100, a nonionic detergent, on the same system. Triton X-100 showed significantly different effect on sucrose-permeability of gap junction channel from that was affected by ginseng saponin. The structures of liposomes containing gap junction channels was significantly destroyed by Triton X-100.

This is a preview of subscription content, log in to check access.

References Cited

  1. Beyer, E. C., Paul, D. L. and Goodenough, D. A., Connexin family of gap junction proteins.J. Membr. Biol., 116, 187–194 (1990).

  2. Ginzberg, R. D. and Gilula, N. B., Modulation of cell junctions during differentiation of the chicken otocyst sensory epithelium,Dev. Biol., 68, 110–129 (1979).

  3. Goodenough, D. A., Paul D. L. and Jesaitis, L., Topological distribution of two connexin32 antigenic sites in intact and split rodent hepatocyte gap junctions.J. Cell Biol., 107, 1817–1824 (1988).

  4. Harris, A. L., Walter, A. and Zimmerberg, J., Transport-specific isolation of large channels reconstituted into lipid vesicles.J. Membr. Biol., 109, 243–250 (1989).

  5. Hertzberg, E. L. and Johnson, R. G.,Gap Junctions. Alan R. Riss, New York, 1985.

  6. Joo, C. N., Several physiological functions and metabolism of ginsenosides in the animal body.Korean J. Ginseng Sci., 17(3), 250–286 (1993).

  7. Laemmli, U. K., Cleavage of structural proteins during the assembly of the head of bacteriohage T4.Nature, 227, 680–686 (1981).

  8. Loewenstein, W. R., Junctional intercellular communication: The cell-to-cell membrane channel.Physiol. Rev., 61, 829–912 (1981).

  9. Loewenstein, W. R., Junctional intercellular communication and the control of growth.Biochim. Biophys. Acta., 560, 1–65 (1979).

  10. Matsunaga, H., Saita, T., Nagumo, F., Mori, M. and Katano, M., Relationship between antiproliperative activity for target acetylenic alcohol, panaxydol, and its affinity for target cell membrane.Japanese J. Cancer & Chemotherapy, 21(15), 2585–2589 (1994).

  11. Murase, K., Yamamoto, T. and Hayashi, K., Nerve growth factor-like immunoreactive substance in Panax ginseng extract.Biosci. Biotech. Biochem., 58(9), 1638–1641 (1994).

  12. Nakade, S., Rhee, S. K., Hamanaka, H. and Mikoshiba, K., Cyclic AMP-dependent phosphorylation of an immunoaffinity-purified homotetrameric IP3 receptor increases Ca2+ influx in reconstituted lipid vesicles.J. Biol. Chem., 269(9), 6735–6742 (1994).

  13. Prpic, V., Green, K. C., Blackmore, P. F. and Exton, J. H., Vasopressin-angiotensin II- and α1-adrenergic-induced inhibition of Ca2+ transport by rat liver plasma membrane vesicles.J. Biol. Chem., 259(3), 1382–1385 (1984).

  14. Rhee, S. K., Paul, D. and Harris, A. L., Purification of connexin32 by monoclonal antibody immunoaffinity chromatography.FASEB J., 3, 2188a (1989).

  15. Sato, K., Mochizuki, M., Saiki, I., Yoo, Y. C., Samukawa, K. and Azuma, I., Inhibition of tumor angiogenesis and mutagenesis by a saponin ofPanax ginseng, ginsenoside-Rb2.Biol. Pharm. Bull., 17(5), 63–639 (1994).

  16. Sheridan, J. D., Cell coupling and cell communication during embryogenesis, In Poste, H. and Nicholson, G. L. (Eds.),The cell surface in animal embryogenesis. Elsevier, New York, 1976, pp. 409–447.

  17. Spray, D. C. and Bennett, M. V. L., Physiology and pharmacology of gap junctions.Annu. Rev. Physiol., 47, 281–303 (1985).

  18. Tang, W. C. and Eisenbrand, G., Chinese drugs of plant origin: chemistry, pharmacology and use in traditional and modern medicine. Vienna: Springer-Verlag (1992).

  19. Trosko, J. E., Chang, C. C. and Metcalf, A., Mechanisms of tumor promotion: potential role of intercellular communication.Cancer Invest., 6, 511–526 (1983).

  20. Yamasaki, H. and Katoh, F., Further evidence for the involvement of gap junctional intercellular communication and maintenance of transformed foci in BALB/c 3T3 cells.Cancer Res., 48, 3490–3495 (1988).

  21. Yokozawa, T., Iwano, M., Dohi, K., Hattori, M. and Oura, H., Inhibitory effect of ginseng on proliferation of cultured mouse mesangial cells.Nippon Jizo, Gakkai Shi., 36(1), 13–18, (1994).

  22. Zhang, T., Hoshino, K., Iguchi, K., Ishikawa, J., Mochizuki, T., Takatsuka, N., Yanaihara, C., Yokota, M., Greeley, G. H. and Yanaihara, N., Ginseng root-evidence for numerous regulatory peptides and insulinotropic activity.Biomed. Res., 11, 49–54 (1990).

Download references

Author information

Correspondence to Seung Keun Rhee.

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Hong, E.J., Huh, K. & Rhee, S.K. Effect of ginseng saponin on gap junction channel reconstituted with connexin32. Arch. Pharm. Res. 19, 264 (1996). https://doi.org/10.1007/BF02976238

Download citation

Key words

  • Ginseng
  • Gap junction channel
  • Connexin32
  • Sucrose-permeability
  • Triton X-100
  • Reconstitution