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Galactosylated chitosan (GC)-graft-poly(vinyl pyrrolidone) (PVP) as hepatocyte-targeting DNA carrier:in vitro transfection

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Galactosylated chitosan-graft-poly(vinyl pyrrolidone) (GCPVP) was synthesized and characterized for hepatocyte-targeting gene carrier. GCPVP itself as well as GCPVP/DNA complex had negligible cytotoxicity regardless of the concentration of GCPVP and the charge ratio, but GCPVP/DNA complex had slightly cytotoxic effect on HepG2 cells only in the case of the higher charge ratio and 20 mM of Ca2+ concentration used. Through the confocal laser scanning microscopy, it is shown that the endocytosis by interaction between galactose ligands of GCPVP and ASGPR of the hepatocytes was the major route of transfection of GCPVP/F-plasmid complexes.

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Correspondence to In-Kyu Park or Hu-Lin Jiang or Seung-Eun Cook or Myung-Haing Cho or Su-ll Kim or Hwan-Jeong Jeong or Toshihiro Akaike or Chong-Su Cho.

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Park, I., Jiang, H., Cook, S. et al. Galactosylated chitosan (GC)-graft-poly(vinyl pyrrolidone) (PVP) as hepatocyte-targeting DNA carrier:in vitro transfection. Arch Pharm Res 27, 1284 (2004). https://doi.org/10.1007/BF02975895

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Key words

  • Galactosylated chitosan
  • Poly(vinyl pyrrolidone)
  • Hepatocyte
  • Gene delivery