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Immunological properties of recombinant hepatitis B surface antigen expressed in mammalian cell (C127)

Abstract

We examined the immunological properties of the recombinant hepatitis B surface antigen (r-HBsAg) which was expressed in mammalian cell (C127). The cross-immunity of r-HBsAg and plasma-derived hepatitis B surface antigen (p-HBsAg) were tested using Western blotting and ELISA with guinea pig polyclonal antibody and naturally infected human-derived antibody and the both antigens show the same results in their response pattern and intensity, which indicate they have a good cross-immunity. From the measurement of ED50 after formalin- or heat-inactivation, both r-HBsAg and p-HBsAg showed ED50 of 0.2∼0.3 in formalin-inactivation, while r-HBsAg was 0.05∼0.09 and p-HBsAg was 0.03∼0.07 in heat-inactivation, which means heat-inactivation method is 3∼4 times superior in immunogenicity. In the immunopersistency test performed in guinea pig for the period of 3 months with two different adjuvants, antibody titer was 34.2 with muramyl dipeptide adjuvant, which was 1.8 times greater than the antibody titer of 18.9 with AlPO4 adjuvant. The mutagenicity of r-HBsAg, examined by Ames test and micronucleus test, were all negative. In conclusion, r-HBsAg has the same cross-immunity with p-HBsAg, and heat-inactivation method and muramyl dipeptide adjuvant allow development of r-HBsAg vaccine with excellent immunogenicity.

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Correspondence to Eung-Chil Choi.

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Lee, Y.S., Kim, B.K. & Choi, E. Immunological properties of recombinant hepatitis B surface antigen expressed in mammalian cell (C127). Arch. Pharm. Res. 21, 543–548 (1998). https://doi.org/10.1007/BF02975372

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Key words

  • Recombinant hepatitis B surface antigen (r-HBsAg)
  • Immunological properties
  • Plasma-derived hepatitis B surface antigen (p-HBsAg)
  • Cross immunity
  • Heat inactivation