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Radiolabelled monoclonal antibodies (McAb): An alternate approach to the conventional methods for the assessment of cardiomyocyte damage in an experimental brain-death pig model


The present study was carried out to determine the possible use of cTn-I in the cardiac myofibrillar architecture, as a potential target forin vivo radioimmunodetection of cardiac damage in a brain death pig model. Radioiodiantion of the anti-cTn-I 5F4 McAb was carried out by lactoperoxidase method. The percentage iodine incorporation achieved was 70∼75%. The radioiodinated McAbs were purified on Sephadex G-25 column and characterised by Paper chromatography, Phast Gel electrophoresis and electroimmunoblotting. Radioiodinated anti-cTn-I 5F4 McAbs were employed alongside Pyrophosphate (Tc99m-PPi) and Thallium201 chloride (Tl201) in 24 landrace pigs (brain-dead=18 & sham-operated=6). The percentage cardiac uptake of the radiolabelled antibody injected dose was significantly higher in the brain dead animals (0.196%) as compared to that of sham-operated animals (0.11%). Specificin vivo localization of radiolabelled McAbs in the infarcted cardiac tissue was confirmed by computer-aided reconstruction of 3-D images of the isolated heart. The preliminary results of the study revealed preferential uptake of radiolabelled antibody at the site of myocyte damage resulting from artificially induced brain death.

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Correspondence to Kh. H. Haider.

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Haider, K.H., Stimson, W.H. Radiolabelled monoclonal antibodies (McAb): An alternate approach to the conventional methods for the assessment of cardiomyocyte damage in an experimental brain-death pig model. Arch. Pharm. Res. 21, 496–502 (1998).

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Key words

  • Brain death
  • Cardiac Troponin-1
  • Monoclonal antibodies
  • Radioimmunoimaging
  • Radioiodination