In the present study, FBL-3 murine erythroleukemia cells were transacted with human OSM(hOSM) gene by recombinant adenovirus, then the immunological properties of hOSM gene-transfected FBL-3 cells(FBL-3-OSM+) were investigated. 4 hours after transfection with hOSM gene, hOSM could be detected in the supernatant of FBL-3-OSM+ cells and hOSM secretion peaked at 24 h. The proliferation of FBL-3-OSM+ cells was inhibited markedly. The clonal formation of FBL-3-OSM+ cells was suppressed more obviously in comparison with wild-type FBL-3 cells when analysed in clonal argar culture. Flow cytometry analysis showed that FBL-3-OSM+ cells expressed higher levels of Fas protein, B7 and ICAM-1 molecuIes.FBL-3-OSM+ cells also expressed higher level of MHC class I molecules(H-2Kb) but remained unchanged in expression of MHC class II molecules (la). CD14, which is a specific marker of monocyte/macrophage and not expressed on the wild-type FBL-3 cells, was also detected on the surface of FBL-3-OSM+ cells. The results suggested that OSM gene transfer could increase the immunogenicity of FBL-3 cells and promote their differentiation into macrophage-like cells. The data outline a promising approach to OSM gene therapy of leukemia mediated by recombinant adenovirus.
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Rose TM and Bruce AG. Oncostatin M is a member of a cytokine family that includes leukemia-inhibitory factor, granulocyte colony-stimulating factor, and interleukin 6. Proc Natl Acad Sci USA 1991; 88:8641.
Wallace PM, Macmaster JF, Rillema JR, et al. In vivo properties of oncostatin M. Ann N Y Acad Sci 1995; 762:42.
Bruce AG, Hoggatt IH, Rose TM. et al. Oncostatin M is a differentiation factor for myeloid leukemia cells. J Immunol 1992; 149:1271.
Yoshida Y. and Hamada H. Adenovirus-mediated inducible gene expression through tetracycline-controllable transactivator with nuclear localization signal. Biochem Biophys Res Commun 1997; 230:426.
Naemura JR and Radka SF. Detection of oncostatin M in human plasma and serum by a sensitive enzyme immunoassay. Lymphokine Cytokine Res 1993; 12:187.
Tanigawa T, Nicola N, McArthur GA, et al. Differential regulation of macrophage differentiation in response to leukemia inhibitory factor/oncostatin-M/interleukin-6: the effect of enforced expression of the SCL transcription factor. Blood 1995; 85:379.
Wallace PM, MacMaster JF, Rillema JR, et al. Thrombocytopoietic properties of oncostatin M. Blood 1995; 86:1310.
Clegg CH, Rulffes JT, Wallace PM, et al. Regulation of an extrathymic T-cell development pathway by oncostatin M. Nature 1996; 384:261.
Kaneda R, Iweabuchi K and Onoe K. Dissociation of Fas-mediated cytotoxicity and FasL expression in a cytotoxic CD4+ T cell clone. Immunol Lett 1997; 55:53.
This work was supported by the National Natural Science Foundation of China (No. 39421009).
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Wang, Q., Cao, X., Mi, J. et al. Immunological characteristics of the leukemia cells transfected with oncostatin M gene by recombinant adenovieus. Chin J Cancer Res 9, 272–276 (1997). https://doi.org/10.1007/BF02974973
- Oncostatin M
- Gene transfer
- Costimulatory molecules