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PAF antagonistic activity of 2-hydroxy-3-methoxybenzoic acid glucose ester fromGentiana scabra

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In order to find out anti-platelet activating factor (PAF) from natural resources, Korean medicinal plants used for the treatments of peripheral circulation disorders were tested for their possible protective effects on PAF-induced anaphylactic shock. From the above screening, the methanol extract ofGentiana scabra showed a potent antagonistic activity against PAF. Water suspension of the extract was partitioned with CH2Cl2 and EtOAc, successively. The EtOAc fraction which showed the highest activity was chromatographed on silica gel to yield 6 fractions. From the fraction which showed higher PAF-antagonistic activity than the other fractions, compound1 was isolated by recrystallization. On the basis of spectral data, compound1 was identified as 2-hydroxy-3-methoxybenzoic acid glucose ester. The compound prevented the mice from the PAF-induced death at a dose of 300 μg/mouse.

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References Cited

  1. Braquet, P., Youqui, L., Shen, T. Y. and Vargaftig, B. B., Perspectives in platelet-activating factor research.Pharmacol. Rev., 39, 97–145 (1987).

  2. Camussi, G., Aglietta, M., Coda, R., Bussolino, F., Piacibello, W. and Tetta, T., Release of platelet-activating factor (PAF) and histamine. II. The cellular origin of human PAF: monocytes, polymorphonuclear neutrophils and basophils.Immunology, 42, 191–199 (1981).

  3. Ha, T., Park, Y. and Rhew, H., Effect of platelet activating factor on fatal active systemic anaphylaxis.Korean J. Immunol., 12, 145–159 (1990).

  4. Herbert, J. M., Lepsy, L. and Maffrand, J. P., Protective effect of SR 27417, a novel PAF antagonist, on lethal anaphylactic and endo-toxin-induced shock in mice.Eur. J. Pharmacol., 205, 271–276 (1991).

  5. Kuo, S-H., Yen, M-H., Chung, M-I. and Lin, C-N., A flavone C-glycoside and an aromatic glucoside from Gentiana species.Phytochem., 41, 309–312 (1996).

  6. Mencia-Huerta, J. M., and Benveniste, J., Platelet-activating factor and macrophage I. Evidence for the release from rat and mouse peritoneal macrophages and not from mastocytes.Eur. J. Immunol., 9, 409–415 (1979).

  7. Siess, W., Molecular mechanisms of platelet activation.Physiol. Rev., 9, 58–178 (1989).

  8. Tahraoui, L., Floch, A., Mondot, S. and Cavero, I., High affinity specific binding sites for tritiated platelet-activating factor in canine platelet membranes: counterparts of platelet-activating factor receptors mediating platelet aggregation.Mol. Pharmacol., 34, 145–151 (1988).

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Correspondence to Hoon Huh.

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Huh, H., Kim, H.K. & Lee, H. PAF antagonistic activity of 2-hydroxy-3-methoxybenzoic acid glucose ester fromGentiana scabra . Arch. Pharm. Res. 21, 436–439 (1998). https://doi.org/10.1007/BF02974639

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Key words

  • Platelet activating factor (PAF)
  • PAF-induced anaphylaxis
  • Gentiana scabra
  • 2-Hydroxy-3-methoxybenzoic acid glucose ester