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Cytométrie en flux et en image: principes et applications

Flow and Image Cytometry: Principles and Applications

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Résumé

La cytométrie en flux et la cytométrie en image permettent une analyse fine des populations cellulaires, des micro-organismes ainsi que des constituants cellulaires. Ces deux techniques sont passées en quelques années d’une utilisation restreinte aux laboratoires de recherche à une utilisation routinière d’aide au diagnostique. Cet article résume les principes, les avantages et les limitations des deux techniques. Il donne aussi un bref aperçu des principales applications en recherche ou en routine.

Summary

Flow and Image Cytometry allow a high precision analysis of cell populations, microorganisms and cell components. Over few years, these two techniques evolved from a restrictive use in research laboratories to a daily use to help diagnosis. This review summarizes the principles, advantages and limitations of these two technologies. It gives an idea on the principal applications in research or clinical diagnosis.

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Duperray, C., Costes, V., Baldet, P. et al. Cytométrie en flux et en image: principes et applications. Acta Endosc 23, 285–294 (1993). https://doi.org/10.1007/BF02970002

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Mots-clés

  • cytométrie en flux
  • cytométrie en image

Key-words

  • flow cytometry
  • image cytometry