We investigated the effect of protein extract ofAsterina pectinifera on the activity of 4 enzymes that may play a role in adenocarcinoma of the colon: quinone reductase (QR), glutathioneS-transferase (GST), ornithine decarboxylase (ODC), and cyclooxygenase (COX)-2. QR and GST activity increased in HT-29 human colon adenocarcinoma cells increased that had been exposed to 4 concentrations of the protein extract (80, 160, 200 and 240 μg/mL). Additionally, 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced ODC activity decreased significantly in cells exposed to the extract in concentrations of 160 μg/mL (p<0.05), 200 μg/mL (p<0.005), and 240 μg/mL (p<0.005). TPA-induced COX-2 activity also decreased in cells exposed to extract concentrations of 10, 20, 40, and 60 μg/mL. COX-2 expression was also inhibited in cells exposed to this extract. These results suggest that this protein extract ofA pectinifera has chemopreventive activity in HT-29 human colon adenocarcinoma cells, and therefore, may have the potential to function as a chemopreventive agent in human colorectal cancer.
This is a preview of subscription content, log in to check access.
Buy single article
Instant access to the full article PDF.
Price includes VAT for USA
Habig, W. H., Pabst, M. J., and Jakoby, W. B., GlutathioneS-transferases: the first enzymatic step in mercapturic acid formation.J. Biol. Chem., 249, 7130–7139 (1974).
Joseph, P., Xie, T., Xu, Y., and Jaiswal, A. K., NAD(P)H:quinone oxidoreductase 1 (DT-diaphorase): expression, regulation, and role in cancer.Oncol. Res., 6, 525–532 (1994).
Kawamori, T., Rao, C. V., Seibert, K., and Reddy, B. S., Chemopreventive activity of celecoxib, a specific cyclooxygenase-2 inhibitor, against colon carcinogenesis.Cancer Res., 58, 409–412 (1998).
Kishimura, H., and Hayashi, K., Isolation and characteristics of trypsin from pyloric ceca of the starfishAsterina pectinifera.Comp. Biochem. Physiol. B., 132, 485–490 (2002).
Liu, X. H., Wiley, H. S., and Meikle, A. W., Androgens regulate proliferation of human prostate cancer cells in culture by increasing transforming growth factor-α (TGF-α) and epidermal growth factor (EGF)/TGF-2 receptor.J. Clin. Endocrinol. Metab., 77, 1472–1478 (1993).
Marnett, L. J. and DuBois, R. N., COX-2: a target for colon cancer prevention.Annu. Rev. Pharmacol. Toxicol., 42, 55–80 (2002).
Marton, L. J. and Pegg, A. E., Polyamines as targets for therapeutic intervention.Annu. Rev. Pharmacol. Toxicol., 35, 55–91 (1995).
Parkin, D. M., Pisani, P., and Ferlay, J., Estimates of the worldwide incidence of eighteen major cancers in 1985.Int. J. Cancer., 54, 594–606 (1993).
Rao, C. V., Simi, B., and Reddy B. S., Inhibition by dietary curcumin of azoxymethane-induced ornithine decarboxylase, tyrosine protein kinase, arachidonic acid metabolism and aberrant crypt foci formation in the rat colon.Carcinogenesis, 14, 2219–2225 (1993).
Rinehart, K. L., Antitumor, compounds from tunicates.Med. Res. Rev., 20, 1–27 (2000).
Ruggieri, G. D., Drugs from the sea.Science, 194, 491–497 (1976).
Schweitzer, J., Handley, F. G., Edwards, J., Harris, W. F., Grever, M. R., Schepartz, S. A., Cragg, G., Snader, K., and Bhat, A., Summary of the workshop on drug development, biological diversity, and economic growth.J. Natl. Cancer Inst., 83, 1294–1298 (1991).
Shon, Y. H. and Nam, K. S., Inhibition of polyamine biosynthesis ofAcanthamoeba castellanii and 12-O-tetradecanoylphorbol-13-acetate-induced ornithine decarboxylase activity by chitosanoligosaccharide.Biotechnol. Lett., 25, 701–704 (2003).
Shon, Y. H., Nam, K. S., and Kim, M. K., Cancer chemopreventive potential ofScenedesmus spp. cultured in medium containing bioreacted swine urine.J. Microbiol. Biotechnol., 14, 158–161 (2004).
Talalay, P., Fahey, J. W., Holtzclaw, W. D., Prestera, T., and Zhang, Y., Chemoprotection against cancer by phase II enzyme induction.Toxicol. Lett. (Amst.) 82, 173–179 (1995).
Tanako, T., Kojima, T., Suzuki, M., and Mori, H., Chemoprevention of colon carcinogenesis by the natural product of a simple phenolic compound protocatechuic acid: suppressing effects on tumor, development and biomarkers expression of colon.Cancer Res., 53, 3909–3913 (1993).
About this article
Cite this article
Shon, Y., Nam, K. Chemopreventive effect of protein extract ofAsterina pectinifera in HT-29 human colon adenocarcinoma cells. Arch Pharm Res 29, 209–212 (2006). https://doi.org/10.1007/BF02969395
- Asterina pectinifera
- Ornithine decarboxylase
- Quinone reductase