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Culture of human peritoneum—A new method to measure the local cytokine response and the effect of immunomodulators

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Summary

The production of cytokines and chemokines, which are involved in cell activation and cell migration in native pieces of peritoneum, was measured to investigate immune regulatory reactions in the human peritoneum. The samples were obtained during abdominal surgery and cultured immediately afterwards. In order to test therapeutic optionsin vitro, the effect of IL-10 and IFN-γ on the cytokine and chemokine production was also studied. The chemokine monocyte chemotactic protein-1 (MCP-1) was produced and released spontaneously. When lipopolysaccharide (LPS) was added, MCP-1 production increased. In addition, TNF-α production was induced by LPS. When IL-10 was added, LPS-stimulated TNF-α production was reduced towards baseline levels, LPS-induced MCP-1 production, but increased baseline MCP-1 production. It can be concluded that short-time culture of native human peritoneum is a method to investigate peritoneal chemokine and cytokine production in patients undergoing abdominal surgery. Further studies are intended to detect cytokine patterns which identify patients at risk of developing peritonitis. In addition, the effects of medications may be testedin vitro in order to investigate options for preventive modulation of the peritoneal immune response in such patients.

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References

  1. 1.

    Christou, N. V.: Predicting infectious morbidity in elective operations. Am. J. Surg. 165 (2A Suppl.) (1993) 52s-58s.

  2. 2.

    Nyström, P. O., Skau, T.: Elimination patterns ofEscherichia coli andBacteroides fragilis from the peritoneal cavity. Studies with experimental peritonitis in pigs. Acta Chir. Scand. 149 (1983) 383–388.

  3. 3.

    Dunn, D. L., Barke, R. A., Knight, N. B., Humphrey, E. W., Simmons, R. L.: Role of resident macrophages, peripheral neutrophils, and translymphatic absorption in bacterial clearance from the peritoneal cavity. Infect. Immun. 49 (1985) 257–264.

  4. 4.

    Dunn, D. L., Barke, R. A., Ewald, D. C., Simmons, R. L.: Macrophages and translymphatic absorption represent the first line of host defense of the periotoneal cavity. Arch. Surg. 122 (1987) 105–110.

  5. 5.

    Skau, T., Nyström, P. O., Ohman, L., Stendahl, O.: The kinetics of peritoneal clearance ofEscherichia coli andBacteroides fragilis and partioipating defense mechanisms. Arch. Surg. 121 (1986) 1033–1039.

  6. 6.

    Skau, T., Nyström, P. O., Ohman, L., Stendahl, O.: Bacterial clearance and granulocyte response in experimental peritonitis. J. Surg. Res. 40 (1986) 13–20.

  7. 7.

    Leak, L. V.: Interaction of mesothelium to intraperitoneal stimulation. I. Aggregation of peritoneal cells. Lab. Invest. 48 (1983) 479–491.

  8. 8.

    Czuprynski, C. J., Brown, J. F.: Purified human and recombinant murine interleukin-1 alpha induced accumulation of inflammatory peritoneal neutrophils and mononuclear phagocytes: possible contributions to antibacterial resistance. Microb. Pathog. 3 (1987) 337–386.

  9. 9.

    Melnicoff, M. J., Horan, P. K., Morahan, P. S.: Kinetics of changes in peritoneal cell populations following acute inflammation. Cell Immunol. 118 (1989) 178–191.

  10. 10.

    Tekstra, J., Visser, C. E., Tuk, C. W., Brower Steenbergen, J. J., Burger, C. W., Krediet, R. T., Beelen, R. H.: Identification of the major chemokines that regulate cell influxes in peritoneal dialysis patients. J. Am. Soc. Nephrol. 7 (1996) 2379–2384.

  11. 11.

    Lanfrancone, L., Boraschi, D., Ghiara, P., Falini, B., Grignani, F., Peri, G., Mantovani, A., Pelicci, P. G.: Human peritoneal mesothelial cells produce many cytokines (granulocyte colony-stimulating factor [CSF], granulocyte-monocyte-CSF, macrophage-CSF, interleukin-1 (IL-1), and IL-6) and are activated and stimulated to grow by IL-1. Blood 80 (1992) 2835–2842.

  12. 12.

    Jonjic, N., Peri, G., Bernasconi, S., Sciacca, F. L., Colotta, F., Pelicci, P., Lafrancone, L., Mantovani, A.: Expression of adhesion molecules and chemotactic cytokines in cultured human mesothelial cells. J. Exp. Med. 176 (1992) 1165–1174.

  13. 13.

    Douvdevani, A., Rapoport, J., Konforty, A., Argov, S., Ovnat, A., Chaimovitz, C.: Human peritoneal mesothelial cells synthesize IL-1 alpha and beta. Kidney Int. 46 (1994) 993–1001.

  14. 14.

    Zhong, W. W., Burke, P. A., Hand, A. T., Walsh, M. J., Hughes, L. A., Forse, R. A.: Regulation of cytokine mRNA expression in lipopolysaccharide-stimulated human macrophages. Arch. Surg. 128 (1993) 158–163; discussion 163–164.

  15. 15.

    Mori, S., Goto, K., Goto, F., Murakami, K., Ohkawara, S., Yoshinaga, M.: Dynamic changes in mRNA expression of neutorphils during the course of acute inflammation in rabbits. Int. Immunol. 6 (1994) 149–156.

  16. 16.

    Seitz, M., Loetscher, P., Dewald, B., Towbin, H., Gallati, H., Baggiolini, M.: Interleukin-10 differentially regulates cytokines inhibitor and chemokine release from blood mononuclear cells and fibroblasts. Eur. J. Immunol. 25 (1995) 1129–1132.

  17. 17.

    Topley, N., Mackenzie, R. K., Williams, J. D.: Macrophages and mesothelial cells in bacterial peritonitis. Immunobiology 195 (1996) 563–573.

  18. 18.

    Nimmanwudipong, T., Cheadle, W. G., Appel, S. H., Polk, H. C. Jr.: Effect of protein malnutrition and immunomodulation on immune cell populations. J. Surg. Res. 52 (1992) 233–238.

  19. 19.

    Christou, N. V., Tellado Rodriguez, J., Chartrand, L., Giannas, B., Kapadia, B., Meakins, J., Rode, H., Gordon, J.: Estimating mortality risk in preoperative patients using immunologic, nutritional, and acute-phase response variables. Ann. Surg. 210 (1989) 67–77.

  20. 20.

    Welsh, F. K., Farmery, S. M., Ramsden, C., Guillou, P. J., Reynolds, J. V.: Reversible impairment in monocyte major histocompatibility complex class II expression in malnourished surgical patients. JPEN. J. Parenter. Enteral Nutr. 20 (1996) 344–348.

  21. 21.

    Jonsson, B., Berglund, J., Skau, T., Nyström, P. O.: Outcome of intra-abdominal infection in pigs depends more on host responses than on microbiology. Eur. J. Surg. 159 (1993) 571–578.

  22. 22.

    Ogle, C. K., Wu, J. Z., Mao, X., Szczur, K., Alexander, J. W., Ogle, J. D.: Heterogeneity of Kupffer cells and splenic, alveolar, and peritoneal macrophages for the production of TNF, IL-1, and IL-6. Inflammation 18 (1994) 511–523.

  23. 23.

    Kubicka, U., Olszewski, W. L., Tarnowski, W., Bielecki, K., Ziolkowska, A., Wierzbicki, Z.: Normal human immune peritoneal cells: subpopulations and functional characteristics. Scand. J. Immunol. 44 (1996) 157–163.

  24. 24.

    Vassalli, P.: The pathophysiology of tumor necrosis factor. Annu. Rev. Immunol. 10 (1992) 411–452.

  25. 25.

    Proost, P., Wuyts, A., Van Damme, J.: Human monocyte chemotactic proteins-2 and-3: structural and functional comparison with MCP-1. J. Leukoc. Biol. 59 (1996) 67–74.

  26. 26.

    Kinnaert, P., De Wilde, J. P., Bournonville, B., Husson, C., Salmon, L.: Direct activation of human peritoneal mesothelial cells by heat-killed microorganisms. Ann. Surg. 224 (1996) 749–754; discussion 754–755.

  27. 27.

    Muller, J., Yoshida, T.: Interaction of murine peritoneal leukocytes and mesothelial cells:in vitro model system to survey cellular events on serosal membranes during inflammation. Clin. Immunol. Immunopathol. 75 (1995) 231–238.

  28. 28.

    Valle, M. T., Degl'Innocenti, M. L., Bertelli, R., Facchetti, P., Perfumo, F., Fenoglio, D., Kunkl, A., Gusmano, R., Manca, F.: Antigenpresenting function of human peritoneum mesothelial cells. Clin. Exp. Immunol. 101 (1995) 172–176.

  29. 29.

    Hailman, F., Vasselon, T., Kelley, M., Busse, L. A., Hu, M. C., Lichenstein, H. S., Detmers, P. A., Wright, S. D.: Stimulation of macrophages and neutrophils by complexes of lipopolysaccharide and soluble CD14. J. Immunol. 156 (1996) 4384–4390.

  30. 30.

    Koerner, T. J., Adams, D. O., Hamilton, T. A.: Regulation of tumor necrosis factor (TNF) expression: interferon-gamma enhances the accumulation of mRNA for TNF induced by lipopolysaccharide in murine peritoneal macrophages. Cell. Immunol. 109 (1987) 437–443.

  31. 31.

    Agosti, J. M., Coombs, R. W., Collier, A. C., Paradise, M. A., Benedetti, J. K., Jaffe, H. S., Corey, L.: A randomized, double-blind, phase I/II trial of tumor necrosis factor and interferon-gamma for treatment of AIDS-related complex (Protocol 025 from the AIDS Clinical Trials Group). AIDS Res. Hum. Retroviruses 8 (1992) 581–587.

  32. 32.

    de Vries, J. E.: Immunosuppressive and anti-inflammatory properties of interleukin 10. Ann. Med. 27 (1995) 537–541.

  33. 33.

    Fuchs, A. C., Granowitz, E. V., Shapiro, L., Vannier, E., Lonnemann, G., Angel, J. B., Kennedy, J. S., Robson, A. R., Affrime, M. B., Cutler, D. L., Grint, P. C.: Clinical, hematologic, and immunologic effects of interleukin-10 in humans. J. Clin. Immunol. 16 (1996) 291–303.

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Haupt, W., Riese, J., Denzel, C. et al. Culture of human peritoneum—A new method to measure the local cytokine response and the effect of immunomodulators. Infection 26, 345 (1998). https://doi.org/10.1007/BF02962269

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Keywords

  • Peritonitis
  • Peritoneal Cavity
  • Mesothelial Cell
  • Chemokine Production
  • Local Immune Response