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Phylogenetic analysis of polyketide synthase genes fromAspergillus ochraceus

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A number of polyketide synthase gene sequences fromAspergillus ochraceus were isolated by both SSH-PCR and degenerate PCR. The deduced amino acid sequences of the corresponding clonedpks DNA fragments were then aligned with the amino acid sequences of other polyketide synthase enzymes. One of thesepks genes is essential for ochratoxin A biosynthesis (OTA-PKS). The OTA-PKS was most similar to methylsalicylic acid synthase (MSAS) type PKS proteins based on the alignment of the ketosynthase domains while if the acyl transferase domains were aligned it appeared to be more similar to PKS enzymes fromCochliobolus heterostrophus. The three PKS proteins identified by degenerate PCR were all from different PKS types, one was a MSAS type enzyme, the second was similar to the PKS proteins involved in lovastatin biosynthesis while the third was not similar to any of the other phylogenetic groupings. Data is presented which suggests that the use of phylogenetic analysis to predict the function of PKS proteins/genes is likely to be significantly enhanced by analyzing more than one domain of the protein.

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  1. 1

    Kuiper-Goodman T, Scott PM (1989) Review: Risk assessment of the mycotoxin ochratoxin A. Biomedical and Environmental Sciences 2: 179–248

  2. 2

    Petzinger E, Ziegler K (2000) Ochratoxin A from a toxicological perspective. J Vet Pharmacol Therap 23: 91–98

  3. 3

    Schlatter C, Studer-Rohr J, Rasonyi T (1996) Carcinogenic and kinetic aspects of ochratoxin A. Food Addit Contam 13: 43–44

  4. 4

    Thuvander A, Paulsen JE, Axberg K, Johansson N, Vidnes A, Enghardt-Barbieri H, Trygg K, Lund-Larsen K, Jahrl S, Widenfalk A, Bosnes V, Alexander J, Hult K, Olsen M (2001) Levels of ochratoxin A in blood from Norwegian and Swedish blood donors and their possible correlation with food consumption. Food Chem Toxicol 39: 1145–1151

  5. 5

    Skaug MA, Helland I, Solvoll K, Saugstad OD (2001) Presence of ochratoxin A in human milk in relation to dietary intake. Food Addit Contam 18: 321–327

  6. 6

    Marquardt RR, Frohlich AA (1992) A review of recent advances in understanding ochratoxicosis. J Anim Sci 70: 3968–3988

  7. 7

    Tatu CA, Orem WH, Finkleman RB, Federer GL (1998) The etiology of Balkan Endemic Nephropathy: still more questions than answers. Environ Health Perspectives 106: 689–700

  8. 8

    Batista LR, Chalfoun SM, Prado G, Schwan RF, Wheals AE (2003) Toxigenic fungi associated with processed (green) coffee beans (Coffea arabica L.). Int J Food Microbiol 85: 293–300

  9. 9

    Battilani P, Pietri A, Bertuzzi A, Languasco L, Giorni P, Kozakiewicz Z (2003) Occurrence of ochratoxin A-producing fungi in grapes grown in Italy. J Food Prot 66: 633–666

  10. 10

    Bayman P, Baker JL, Doster MA, Michaillides TJ, Mahony NE (2002) Ochratoxin production by theAspergillus ochraceus group andAspergillus alliaceus. Appl Environ Microbiol 68: 2326–2329

  11. 11

    Jorgensen K (1998) A survey of pork, poultry, coffee, beer and pulses for Ochratoxin A. Food Addit Contam 15: 550–554

  12. 12

    Jorgensen K, Jacobsen JS (2002) Occurrence of ochratoxin A in Danish wheat and rye, 1992–1999. Food Addit Contam 19: 1184–1189

  13. 13

    Gareis M, Scheuer R (2000) Ochratoxin A in meat and meat products. Arch Lebensmittelhyg 51: 102–104

  14. 14

    Petzinger E, Weidenbach A (2002) Mycotoxins in the food chain: the role of ochratoxins. Livestock Prod Sci 76: 245–250

  15. 15

    Scudamore KA, Banks J, McDonald KJ (2003) The fate of ochratoxin A in the processing of whole wheat grains during milling and bread production. Food Addit Contam 20: 1153–1163

  16. 16

    Odhav B, Naicker V (2002). Mycotoxins in South-African traditionally brewed beers, Food Addit Contam 19: 55–61

  17. 17

    Pitt JI (2000) Toxigenic fungi: which are important? Med Mycol 38 (Suppl.): 17–22

  18. 18

    Bau M, Bragulat MR, Abarca ML, Minguez S, Cabanes FJ (2005) Ochratoxigenic species from Spanish wine grapes. Int J Food Microbiol 98: 125–130

  19. 19

    Abarca ML, Bragulat MR, Castella G, Cabanes FJ (1994) Ochratoxin A production by strains ofAspergillus niger var.niger. Appl Environ Microbiol 60: 2650–2652

  20. 20

    Schuster E, Dunn-Coleman N, Frisvad JC, Van Dijck PW (2002) On the safety ofAspergillus niger — a review. Appl Microbiol Biotechnol 59: 426–435

  21. 21

    Castella G, Larsen TO, Cabanes J, Schmidt H, Alboresi A, Niessen L, Farber P, Geisen R (2002) Molecular characterization of ochratoxin A producing strains of the genus Penicillium. Syst Appl Microbiol 25: 74–83

  22. 22

    Lund F, Frisvad JC (2003)Penicillium verrucosum in wheat and barley indicates the presence of ochratoxin A. J Appl Microbiol 95: 1117–1123

  23. 23

    O'Callaghan J, Caddick MX, Dobson ADW (2003) A polyketide synthase gene required for ochratoxin A biosynthesis inAspergillus ochraceus. Microbiol 149: 3485–3491

  24. 24

    Varga J, Rigo K, Kocsube S, Farkas B, Pal K (2003) Diversity of polyketide synthase gene sequences inAspergillus species. Res Microbiol 154: 596–600

  25. 25

    Nicholson TP, Rudd BAM, Dawson M, Lazarus CM (2001) Design and utility of oligonucleotide gene probes for fungal polyketide synthases. Chem Biol 8: 157–78

  26. 26

    Higgins D, Thompson J, Gibson T, Thompson JD, Higgins DG, Gibson TJ (1994) CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22: 4673–4680

  27. 27

    Felsenstein J (1989) PHYLIP — Phylogeny Inference Package (Version 3.2). Cladistics 5: 164–166

  28. 28

    Hopwood D (1997) Genetic contributions to understanding polyketide synthases. Chem Rev 97: 2465–2497

  29. 29

    Moore BS, Hopke BN (2001) Discovery of a new bacterial polyketide biosynthetic pathway. Chembiochem 2: 35–8

  30. 30

    Shen B, Hutchinson CR (1996) Deciphering the mechanism for the assembly of aromatic polyketides by a bacterial polyketide synthase. Proc Natl Acad Sci U S A. 93: 6600–6604

  31. 31

    Marahiel MA, Stachelhaus T, Mootz HD (1997) Modular peptide synthases involved in nonribosomal peptide synthesis. Chem Rev 97: 2651–2674

  32. 32

    Bingle LE, Simpson TJ, Lazarus CM (1999) Ketosynthase domain probes identify two subclasses of fungal polyketide synthase genes. Fungal Genet Biol 26: 209–223

  33. 33

    Fujii I (1998) Polyketide biosynthesis in filamentuous fungi. In: Sankawa U (ed) Comprehensive Natural products Chemistry, Elsevier, Amsterdam, 409–441

  34. 34

    Kroken S, Glass NL, Taylor JW, Yoder OC, Turgeon BG (2003) Phylogenomic analysis of type I polyketide synthase genes in pathogenic and saprobic ascomycetes. Proc Natl Acad Sci USA. 100: 15670–15675

  35. 35

    Harris JP, Mantle PG (2001) Biosynthesis of ochratoxins byAspergillus ochraceus. Phytochem 58: 709–716

  36. 36

    Durley RC, Macmillan J, Simpson TJ, Glen AT, Turner WB (1975) Fungal products. Part XIII. Xanthomegnin, viomellein, rubrosulphin, and viopurprin, pigments from Aspergillus sulphureus and Aspergillus melleus. J Chem Soc Perkin 1975: 163–169

  37. 37

    de Guzman FS, Bruss DR, Rippentrop JM, Gloer KB, Gloer JB, Wicklow DT, Dowd PF (1994) Ochrindoles A-D: New bis-indolyl benzenoids from the sclerotia ofAspergillus ochraceus NRRL 3519. J Nat Prod 57: 634–639

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Correspondence to A. D. W. Dobson.

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Financial support: Irish Government under the National Development Plan 2000–2006

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O'Callaghan, J., Dobson, A.D.W. Phylogenetic analysis of polyketide synthase genes fromAspergillus ochraceus . Mycotox Res 22, 125–133 (2006). https://doi.org/10.1007/BF02956776

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  • Aspergillus ochraceus
  • ochratoxin
  • polyketide synthases