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Molecular and chemical monitoring of growth and ochratoxin a biosynthesis ofP. verrucosum in wheat stored at different moisture conditions

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Abstract

The growth and ochratoxin A producing activity at the phenotypical and molecular level ofP. verrucosum in wheat stored at different relative moisture conditions have been followed. Growth has been measured by determination of colony forming units (cfu). The expression of the ochratoxin A polyketide synthase (otapksPV) has been followed over time by reverse transcriptase Real Time PCR, whereas the biosynthesis of ochratoxin A by the fungus has been followed by HPLC. The remoisted and inoculated wheat was kept in perforated plastic bags and stored in tower silos. The bags were adjusted to 24%, 19% and 14% (m/m) relative moisture and stored for 6 month (September 06 - February 06) at ambient temperatures. A high increase of the cfu number up to a level of about lx108 cfu/g could be observed in the 24% sample. Interestingly no growth could be detected in the 14% and 19% samples. This result was supported by the quantitative Real Time PCR data. In congruence with this growth behaviour ochratoxin A could be detected from the first to the last time point in the 24% sample. Interestingly the ochratoxin A amounts measured over time did not show increasing or constant values, but varied from higher to lower values. After storage of three month (November 2006) the ochratoxin A concentration in the wheat was highest. The expression of theotapksPV gene mirrors this behaviour. The expression of this gene also fluctuates around a certain value, showing high activity after three month of storage.

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References

  1. 1

    Petzinger E, Ziegler K (2000) Ochratoxin A from a toxicological perspective. J Vet Pharmacol Therap 23: 91–98

  2. 2

    European Commission (2006) Commission Regulation (EC) No 1886/2006 of 19 December 2006 setting maximum levels for certain contaminants in foodstuffs. OJ L3 64: 5–24

  3. 3

    Lund F, Frisvad JC (2003)Penicillium verrucosum in wheat and barley indicates presence of ochratoxin A. J Appl Microbiol 95: 1117–1123

  4. 4

    Bucheli P, Kanchanomai C, Meyer I, Pittet A (2000) Development of Ochratoxin A during Robusta (Coffea canephora) Coffee Cherry Drying. J Agric Food Chem 48: 1358–1362

  5. 5

    Abarca ML, Accensi F, Bragulat MR, Castella G, Cabanes FJ (2003)Aspergillus carbonarius as the main source of ochratoxin A contamination in dried vine fruits from the Spanish market. J Food Protect 66: 504–506

  6. 6

    Thirumala-Devi K, Mayo MA, Reddy G, Emmanuel KE, Larondelle Y, Reddy DVR (2001) Occurrence of ochratoxin A in black pepper, coriander, ginger and turmeric in India. Food Addit Contam 18 (9): 830–835

  7. 7

    Gareis M (1996) Fate of ochratoxin A on processing of meat products. Food Addit Contam 13: 35–37

  8. 8

    Mantle PG (1998) Ochratoxin A in Coffee. J Food Mycol 2: 63–65

  9. 9

    Frisvad J, Frank JM, Houbraken JAMP, Kuijpers AFA, Samson RA (2004) New ochratoxin A producing species ofAspergillus sectionCircumdati. Stud Mycol 50: 23–43

  10. 10

    Varga J, Kozakiewicz Z (2006) Ochratoxin A in grapes and grape-derived products. Trends Food Sci Tech 17: 72–81

  11. 11

    Larsen TO, Svendsen A, Smedsgaard J (2001) Biochemical characterization of ochratoxin Aproducing strains of the genusPenicillium. Appl Environ Microbiol 67: 3630–3635

  12. 12

    Castella G, Larsen TO, Cabanes J, Schmidt H, Alboresi A, Niessen L, Färber P, Geisen R (2002) Molecular characterization of ochratoxin A producing strains of the genusPenicillium. System Appl Microbiol 25: 74–83

  13. 13

    Bogs C, Battilani P, Geisen R (2006) Development of a molecular detection and differentiation system for ochratoxin A producingPenicillium species and its application to analyse the occurrence ofPenicillium nordicum in cured meats. Int J Food Microbiol 107: 39–47

  14. 14

    Cholmakov-Bodechtel C, Wolff J, Gareis M, Bresch H, Engel G, Majerus P, Rosner H, Schneider R (2000) Ochratoxin A: Representative food consumption survey and epidemiological analysis. Arch Lebensmittelhyg 51: 111–117

  15. 15

    Lindblad M, Johnsson P, Jonsson N, Lindqvist R, Olsen M (2004) Predicting noncompliant levels of ochratoxin A in cereal grain fromPenicillium verrucosum counts. J Appl Microbiol 97: 609–616

  16. 16

    Olsen M, Jonsson N, Magan N, Banks J, Fanelli C, Rizzo A, Haikara A, Dobson A, Frisvad J, Holmes S, Olkku J, Persson S-J, Börjesson T (2006) Prevention of ochratoxin A in cereals in Europe. In: Hocking AD, Samson RA, Pitt JI, Thrane U (eds) Advances in Food Mycology. Springer Verlag, Heidelberg, 317–342

  17. 17

    Anonymous (2005) -Grown Cereals Authority, www.hgca.com

  18. 18

    Gareis M, Rodel W (2002) Produktion von Ochratoxin A und B durchPenicillium verrucosum auf Gerste in Abhängigkeit der Parameter Wasseraktivität, Wassergehalt und Temperatur. Mycotoxin Research 18A: 132–137

  19. 19

    Schmidt-Heydt M, Richter W, Michulec M, Buttinger G, Geisen R (2007) A comprehensive molecular system to study presence growth and ochratoxin A biosynthesis ofP. verrucosum in wheat. Food Addit Contam (submitted)

  20. 20

    Abramson D, Richter W, Rintelen J, Sinha RN, Schuster M (1992) Ochratoxin A production in Bavarian cereal grains stored at 15 and 19% moisture content. Arch Environ Contam Toxicol 23: 259–265

  21. 21

    Schmidt-Heydt M, Geisen R (2007) Gene expression as an indication for ochratoxin A biosynthesis inPenicillium nordicum. Mycotoxin Research 23: 13–21

  22. 22

    Geisen R (2004) Molecular monitoring of environmental conditions influencing the induction of ochratoxin A biosynthetic genes inPenicillium nordicum. Mol Nutr Food Res 48: 532–540

  23. 23

    European Committee for Standardization (2003) Foodstuffs - Determination of ochratoxin A in barley and roasted coffee - HPLC method with immunoaffinity column clean - up. European Standard EN 14132:2003

  24. 24

    Cairns-Fuller V, Aldred D, Magan N (2005) Water, temperature and gas composition interactions affect growth and ochratoxin A production by isolates ofPenicillium verrucosum on wheat grain. J Appl Microbiol 99: 1215–1221

  25. 25

    Schmidt-Heydt M, Baxter E, Geisen R, Magan N (2007A) Physiological relationship between food preservatives, environmental factors, ochratoxin andotapksPV gene expression byP. verrucosum. Int J Food Microbiol (accepted)

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Author information

Correspondence to M. Schmidt-Heydt or W. Richter or M. Michulec or G. Buttinger or R. Geisen.

Additional information

Financial support: Part of the work was supported by the EU project: “Development of cost-effective control and prevention strategies for emerging and future food borne pathogenic microorganisms throughout the food chain” (PathogenCombat FOOD-CT-2005-07081)

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Schmidt-Heydt, M., Richter, W., Michulec, M. et al. Molecular and chemical monitoring of growth and ochratoxin a biosynthesis ofP. verrucosum in wheat stored at different moisture conditions. Mycotox Res 23, 138–146 (2007). https://doi.org/10.1007/BF02951510

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Keywords

  • P. verrucosum
  • ochratoxin A
  • relative moisture
  • polyketide synthase gene expression