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Fructose production

Coimmobilized amyloglucosidase, pullulanase, and glucose isomerase on BIOBONETM


Amyloglucosidase, pullulanase, and glucose isomerase were coimmobilized onto granular chicken bone (BIOBONETM). Enzyme ratios showing optimum glucose and fructose production (0.7:10:22.3 U amyloglucosidase: pullulanase: glucose isomerase) resulted in 14.4±1.9% of activity bound relative to an equal amount of free enzyme. The estimated specific activity for these enzymes decreased 4.6-fold with immobilization. ReactionpH strongly influenced the yield and ratio of glucose and fructose produced. Net hexose production from the immobilized system was optimal atpH 6.5 and 55°C with a fructose yield of about 20%.

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Correspondence to Doris Y. Schafhagser or Kenneth B. Storey.

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Schafhagser, D.Y., Storey, K.B. Fructose production. Appl Biochem Biotechnol 36, 63–74 (1992). https://doi.org/10.1007/BF02950775

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Index Entries

  • Amyloglucosidase
  • chicken bone
  • pullulanase
  • enzyme coimmobilization
  • glucose isomerase
  • high fructose syrup