Springer Nature is making SARS-CoV-2 and COVID-19 research free. View research | View latest news | Sign up for updates

Effect ofFoilum mori on adipocyte differentiation


Several natural products were tested to control the differentiation of cultured human mesenchymal stem cell into adipocyte. The extent of the inhibitory effect on the conversion of adipose was measured using Oil red O staining, which stains accumulated lipid droplets in the cytoplasm of adipocyte. Of the various natural product extracts, the adipocyte differentiation was inhibited by an extract fromFolium mori in the concentration range 1×10−4∼5×10−2 g/mL. These results suggest thatFolium mori has an inhibitory activity toward the adipose conversion, which is a major cause of obesity.

This is a preview of subscription content, log in to check access.


  1. [1]

    Pittenger, M. F. and D. R. Marshak (1999), Multilineage potential of adult Human mesenchymal stem cells.Science 284: 143–147.

  2. [2]

    Ailhaud, G., P. Grimaldi, and R. Negrel (1992), Cellular and molecular aspects of adipose tissue development.Annu. Rev. Nutr. 12: 207–233.

  3. [3]

    Ramirez-Zacarias, J. L. and W. Kuri-Harcuch (1992) Quantitation of adipose conversion and triglycerides by staining intracytoplasmic lipids with Oil red O.Biochemistry 97: 493–497.

  4. [4]

    Sottile, V. and K. Seuwen (2000), Bone morphogenetic protein-2 stimulates adipogenic differentiation of mesenchymal precursor cells in synergy with BRL 49653 (rosiglitazone).FEBS Lett. 475: 201–204.

  5. [5]

    Wise, L. S. and H. Green (1979), Participation of one isozyme of cytosolic glycerophosphate dehydrogenase in the adipose conversion of 3T3 cells.J. Biol. Chem. 254: 273–275.

  6. [6]

    Kuri-Harcuch, W. and H. Green (1978) Interruption of the adipose conversion of 3T3 cells by biotin deficiency: differentiation without triglyceride accumulation.Cell 14: 53–59.

  7. [7]

    Kasturi R. and V. C. Joshi (1982) Hormonal regulation of stearoyl coenzyme A desaturase activity and lipogenesis during adipose conversion of 3T3-L1 cells.J. Biol. Chem. 257: 12224–12230.

  8. [8]

    Murray, T. and T. R. Russell (1980) Inhibition of adipose conversion in 3T3-L2 cells by retionoic acid.J. Supramole. Structure 14: 255–266.

  9. [9]

    Ignotz, R. A. and J. Massague (1985) Typeß transforming growth factor controls the adipogenic differentiation of 3T3 fibroblasts.Proc. Nat. Acad. Sci. USA 82: 8530–8534.

  10. [10]

    Kawada, T., N. Aoki, Y. Kamei and E. Sugimoto (1990) Comparative investigation of vitamins and their analogues on terminal differentiation, from preadipocytes to adipocytes, of 3T3-L1 cells.Comp. Biochem. Physiol. 96A: 323–326.

  11. [11]

    Hayashi, I., T. Nixon, M. Morikawa, and H. Green (1981) Adipogenic and anti-adipogenic factors in the pituitary and other organs.Proc. Nat. Acad. Sci. USA 6: 3969–3972.

  12. [12]

    Miriam J. S., L. S. Rodney, and W. Walker (1993) Proadipocyte cell lines: Models of cellular proliferation and differentiation.J. Cell Sci. 106: 1–9.

  13. [13]

    Ahn M. Y. and Y. K. Lee (2001) Cytotoxicity of the ethanol extracts of mulberry leaves, branches and silkworm feces.Korean J. Seric. Sci. 43: 26–28.

  14. [14]

    Lee K. H. and S. H. Chung (2000) Antidiabetic effect and mechanism of Mori Folium on streptozotocin-induced diabetic mouse.Bull. K. H. Pharma. Sci. 28: 87–99.

  15. [15]

    Pascot, A. (2001) Deterioration of the metabolic risk profile in women: Respective contributions of impaired glucose tolerance and visceral fat accumulation.Diabetes Care 24: 902–908.

Download references

Author information

Correspondence to Sang Yo Byun.

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Lee, G.W., Byun, S.Y. Effect ofFoilum mori on adipocyte differentiation. Biotechnol. Bioprocess Eng. 8, 299–302 (2003).

Download citation


  • adipocyte
  • Folium mori
  • adipose conversion