Advertisement

Springer Nature is making SARS-CoV-2 and COVID-19 research free. View research | View latest news | Sign up for updates

c-erbB-2 oncoprotein expression in malignant and nonmalignant breast tissue

Summary

Using a specific polyclonal antibody (21) to the c-erbB-2 oncoprotein, a total of 200 primary breast carcinomas of various histological types and 46 benign breast lesions, were immunohistochemically stained, to identify those tissues over-expressing this putative transmembrane receptor. Positivity for this oncoprotein was evident as an intense brown granular staining predominantly located at the cell membrane and was present in 17% (n=34) of the primary breast carcinomas investigated. With the exception of one case of apocrine metaplasia, none of the remaining benign breast samples (n=45) were positive for this oncoprotein. This study shows that overexpression of the c-erb B-2 oncoprotein is easily identifiable and can be detected on paraffin embedded sections using an immunohistochemical technique. The results obtained correlate well with other c-erbB2 detection systems and in addition, positivity for this oncoprotein is mostly confined to the malignant phenotype.

This is a preview of subscription content, log in to check access.

References

  1. 1.

    Escot, C., Theillet, C., Lidereau, R., et al. Genetic alteration of the c-myc protooncogene (MYC) in human primary breast carcinomas. Proc. Natl. Acad. Sci. USA 1986:83, 4834–4838.

  2. 2.

    Mariani-Costantini, R., Escot, C., Thelliet, C., et al. In situ c-myc expression and genomic status of the c-myc locus in infiltrating ductal carcinomas of the breast. Cancer Res. 1988:48, 199–205.

  3. 3.

    Ro, J., North, S.M., Gallick, G.E., Hortobagyi, G.N., Gutterman, Ju. and Blick, M. Amplified and overexpressed epidermal growth factor receptor gene in uncultured primary human breast carcinomas. Cancer Res. 1988:48, 161–164.

  4. 4.

    Slamon, D.J., Clark, G.M., Woing, S.G., Levin, W.J. Ullrich, A. and McGuire, W.L. Human breast cancer: Correlation of relapse and survival with amplification of the HER-2/neu oncogene. Science 1987:235, 177–182.

  5. 5.

    Berger, M.S., Locher, G.W., Saurer, S., et al Correlation of c-erbB-2 gene amplification and protein expression in human breast carcinoma with nodal status and nuclear grading. Cancer Res. 1988:48, 1238–1243.

  6. 6.

    Zhou, D., Battifora, H., Yokota, J., Yamamoto, T. and Cline, M.J. Association of multiple copies of the c-erbB-2 oncogene with spread of breast cancer. Cancer Res. 1987:47, 6123–6125.

  7. 7.

    King, C.R., Kraus, M.H. and Aaronson, S.A. Amplification of a novel v-erb Brelated gene in a human mammary carcinoma. Science 1985: 229, 974–976.

  8. 8.

    Kraus, M.H., Popescu, N.C., Amsbaugh, S.C. and King, C.R. Overexpression of the EGF receptor-related protooncogene erb-B2 in human mammary tumour cell lines by different molecular mechanisms. EMBOJ, 1987:6, 605–610.

  9. 9.

    Schechter, A.L., Hung.M.C. Vaidyanathan, L. et al. A neu gene: and erb B-homologous gene distinct from and unlinked to the gene encoding the EGF receptor. Nature (Lond) 1985:229, 976–978.

  10. 10.

    Schechter, A.L., Stem, D.F., Vaidyanathan, L. et al. A neu oncogne: An erb B-related gene encoding a 185,000 - M tumour antigen. nature (Lond) 1984:312, 513–516.

  11. 11.

    Bargmann, C.I. Hung, M.C. and Weinberg, R.A. Multiple independent activiations of the neu oncogene by a point mutation altering the transmembrane domain of p185. Cell. 1986: 45, 649–657.

  12. 12.

    Coussens, L., I. Yang-Feng, Y.L. Liao, Y.C. et al. Tyrosine kinase receptor with extensive homology to EGF receptor shares chromosomal location with neu oncognee. Science (Wash DC) 1985:230, 1132–1185.

  13. 13.

    Fukushige, S., Matsubaru, K., Yoshida, M. et al. Localization of a novel v-erb B-related Gene, c-erbB-2 on human chromosome-17 and its amplification in a gastric cell line. Mol. Cell. Biol. 1986:6, 955–958.

  14. 14.

    Yamamoto, T., Ikawa, S., Akiyama, T. et al. Similarity of protein encoded by human c-erbB-2 gene to epidermal growth factor receptor. Nature (Lond.) 1986:319, 230–235.

  15. 15.

    Bargmann, C.I., Hung, M.C. and Weiberg, R.A. The neu oncogene encodes an epidermal growth factor receptor-related protein. Nature (Lond.) 1986: 319, 226–230.

  16. 16.

    Yokota, J., Terada, M., Toyoshima, K. et al. Amplification of the C-erb B2 oncogene in human adenocarcinomas in vivo. Lancet 1986: 1, 765–767.

  17. 17.

    Tal., M., Wetzler, M., Josefberg, A. et al. Sporadic amplification of the HER 2/neu protooncogene in adenocarcinomas of various Tissues. Cancer Res. 1988: 48, 1517–1520.

  18. 18.

    Semba, K., Kamata, N., Toyoshima, K. and Yamamoto, T.A. v-erb B-related protooncogene, C-erb B2, is distinct from the C-erb B1/Epidermal growth factor gene and is amplified in a human salivary gland tumour. Proc. Natl. Acad. Sci. (USA. DC) 1985: 82, 6497–6501.

  19. 19.

    Gullick, W.J. Berger, M.S., Bennett, P.L.P., Rothbard, J.B. and Waterfield M.D. Expression of the c-erbB-2 protein in normal and transformed cells. Int. J. Cancer 1987: 40, 246–254.

  20. 20.

    Venter, D.J. Tuzi, N.L. Kumar, S. and Gullick, W.J. Overexpression of the c-erb B-2 oncoprotein in human breast carcinomas immunohistological assessment correlates with gene amplification. Lancet 1987: 2, 69–72.

  21. 21.

    Gusterson, B.A., Gullick, W.J., Benter, D.J. et al. Immunohistochemocal localisation of c-erbB-2 in human breast carcinomas, Mol. Cell Probes 1987: 1, 383–391.

  22. 22.

    Hsu, S.M., Raine, L. and Fanger, H. The Use of Avidin-biotin-peroxidase Complex (ABC) in Immunioperoxidase Technique: A comparison between ABC and unlabelled antibody (PAP) procedures J. Histochem. Cytochem. 1981: 29, 577–580.

  23. 23.

    Di Fiori, P.P. Pierce, J.H., Kraus, M.H., Segato, O., King, C.R. and Aaronson S.A. erb B2 is a potent oncogene when overexpressed in NIH 3T3 Cells. Science 1987: 237, 178- 182.

  24. 24.

    Hudziak, R.M., Schlessinger, J. and Ullrich, A. Increased expression of the putative growth factor receptor p185HER2 causes transformation and tunourigenesis of NIH 3T3 Cells. Proc. Natl. Acad. Sci. USA 1987: 6, 3341–3351.

  25. 25.

    Akiyama, T., Sudo, C., OPgawara, H., Toyoshima, K. and Yamamoto, T. The product of the human c-erbB-2 gene. A 185-kilodalton glycoprotein with tyrosine kinase activity. Science 1986: 232, 1644–1646.

Download references

Author information

Correspondence to A. McCann or P. A. Johnston or P. A. Dervan or W. J. Gullick or D. N. Carney.

Rights and permissions

Reprints and Permissions

About this article

Cite this article

McCann, A., Johnston, P.A., Dervan, P.A. et al. c-erbB-2 oncoprotein expression in malignant and nonmalignant breast tissue. I.J.M.S. 158, 137–140 (1989). https://doi.org/10.1007/BF02943053

Download citation

Keywords

  • Epidermal Growth Factor Receptor
  • Human Epidermal Growth Factor Receptor
  • Epidermal Growth Factor Receptor Gene
  • Primary Breast Carcinoma
  • Benign Breast Lesion