Springer Nature is making SARS-CoV-2 and COVID-19 research free. View research | View latest news | Sign up for updates

Evaluation of rapid immunochromatographic assay kit for HBsAg-screening using whole blood

  • 37 Accesses


A rapid immunochromatographic assay kit using whole blood to screen hepatitis B surface antigen was developed and evaluated by using sera from 240 patients. The reference diagnosis was based on the results obtained with GENEDIA Anti-HBs Rapid kit which is very similar to the above kit except for the use of serum. The test demonstrated a good correlation with the reference immunochromatographic assay kit, that is, the sensitivity and the specificity of the kit was 100%, respectively. The rapid test kit using whole blood should be more convenient and useful for the diagnosis of hepatitis B virus because the kit does not need machines and time to prepare serum. In addition, this kit is safe from inadvertent infection during sample treatment because the blood is sterilized with hydrogen peroxide, eliminates the procedure required to prepare serum and reduces the possibility of exposure to infectious agents.

This is a preview of subscription content, log in to check access.


  1. [1]

    Klingenberg, K. D. V. and J. Espandiari (1996) Evaluation of a one-step test for rapid, in practice detection of rota-virus in farm animals.Veterinary Record. 138: 393–395.

  2. [2]

    Laitinen, M. P. and M. Vuento (1996) Immunochromatographic assay for quantitation of milk progesterone.Acta. Chem. Scand. 50: 141–145.

  3. [3]

    Sato, K., S. Ichiyama, Y. Iinuma, T. Nada, K. Shimokata, and N. Nakashima (1996) Evaluation of immunochromatographic assay systems for rapid detection of hepatitis B surface antigen and antibody. Dainascreen HBsAg and Dainascreen Ausal.J. Clin. Microbiol. 34: 1420–1422.

  4. [4]

    Lou, S. C., C. Patel, S. Ching, and J. Gordon (1995) One-step competitive immunochromatographic assay for semi-quantitative determination of lipoprotein(a) in plasma.Clin. Chem. 39: 619–624.

  5. [5]

    Habib, M. P., R. B. Schifman, B. Y. Shon, J. F. Fiastro, and S. C. Campbell (1987) Evaluation of whole blood theophylline enzyme immunochromatography assay.Chest. 92: 129–131.

  6. [6]

    Shin, H. S., Y. B. Kim, J. W. Shin, C. K. Kim, W. S. Lee, H. K. Kim, K. H. Chung, and J. W. Kim (1997) The evaluation of immunochromatographic assay kit for rapid detection of hepatitis B surface antigen.J. Kor. Soc. Virol. 27(2): 137–141.

  7. [7]

    Karuzina, I. I., G. I. Bachmanova, and A. I. Archakov (1995) Self-inactivation of cytochrome P-450 in the catalytic cycle.Vestn. Ross. Akad. Med. Nauk. 2: 17–29.

  8. [8]

    Schwartz, C. E., J. Krall, L. Norton, K. McKay, D. Kay, and R. E. Lynch (1983) Catalase and superoxide dismutase inEscherichia coli.J. Biol. Chem. 258: 6277–6281.

  9. [9]

    Warren, S. B. and B. N. Tomas (1980)Designing a New Study, pp. 87–89. Williams and Wilkins, Baltimore, USA.

  10. [10]

    Lee K. B. (1991)Clinical Pathology Handbook, pp. 188. Gomoon-sa, Seoul, Korea.

  11. [11]

    Linder, E. and A. Miettinen (1976) Prozone effects in indirect immunofluorescence.Scand. J. Immunol. 5: 513–519.

  12. [12]

    Flynn, N., S. Jain, E. M. Keddie, J. R. Carlson, M. B. Jennings, H. W. Haverkos, N. Nassar, R. Anderson, S. Cohen, and D. Goldberg (1994)In vitro activity of readily available household materials against HIV-1: Is bleach enough?J. AIDS. 7: 747–753.

Download references

Author information

Correspondence to Hyeong-Soon Shin.

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Shin, H., Heo, T. Evaluation of rapid immunochromatographic assay kit for HBsAg-screening using whole blood. Biotechnol. Bioprocess Eng. 5, 362–365 (2000). https://doi.org/10.1007/BF02942213

Download citation


  • HBsAg
  • immunochromatographic assay
  • whole blood
  • sensitivity
  • specificity