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Characterization of the purified extracellulard-xylose isomerase devoid ofd-glucose isomerase fromChainia sp.

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Abstract

Chainia sp. (NCIM 2960), an actinomycete, produced an extracellular, specificd-xylose isomerase and ad-glucose isomerase, along with the conventional intracellular nonspecificd-glucose (xylose) isomerase. The extracellular, specificd-xylose isomerase devoid ofd-glucose isomerase activity was purified 11.2 fold and found to be homogeneous, as observed on sodium dodecyl sulphate polycrylamide gel electrophoresis and disk gel electrophoresis at pH 7.5 and 8.3. The average mol wt of the enzyme, as determined by three different methods, is 71,000 dalton. Optimum temperature, pH, and pI of the enzyme are 60°C, 9.5, and 3.55, respectively. The enzyme, in the presence of Mg2+ (5 mM), catalyzes isomerization ofd-xylose tod-xylulose, but has no action either ond-glucose ord-ribose. Hg2+, Cu2+, Sn2+, and EDTA, xylitol, mannitol, and sorbitol (5 mM each) were inhibitory to the enzyme activity. The equilibrium ratio ofd-xylose andd-xylulose in the reaction mixture was influenced by BO 3 3− and temperature. Chemical modifiers for arginine (phenylglyoxal, 2,3-butanedione) and histidine (diethylpyrocarbonate) were inhibitory tod-xylose isomerase activity, suggesting their involvement in the reaction mechanism of the enzyme.

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Correspondence to A. H. Lachke.

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NCL communication number 4428.

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Khire, J.M., Lachke, A.H., Srinivasan, M.C. et al. Characterization of the purified extracellulard-xylose isomerase devoid ofd-glucose isomerase fromChainia sp.. Appl Biochem Biotechnol 23, 41 (1990). https://doi.org/10.1007/BF02942051

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Index Entries

  • Extracellular isomerase
  • xylose isomerase
  • Chainia sp.
  • glucose isomerase