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Application of a PCR protocol for the diagnosis of trichothecene producingFusarium species in deoxynivalenol contaminated wheat

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Abstract

Trichothecenes pose a health risk to consumers and the presence of the producing fungi causes problems in industrial processing of cereals. A Triplex PCR was developed for screening of samples contaminated with toxigenic Fusarium species. The Triplex PCR could detect Fusarium species with high precision as compared to microbiological data of sample materials. There was a positive correlation between the occurrence of a PCR signal and the prevalence of the mycotoxin DON (Deoxynivalenol).

Zusammenfassung

Trichothecene stellen ein Gesundheitsrisiko für Verbraucher dar und das Auftreten der Pilze, die diese Toxine produzieren, führt zu Problemen in der industriellen Verarbeitung von Getreide. Für die Untersuchung von Proben, die mit toxinbildenden Fusarium-Arten kontaminiert sind, wurde eine Triplex-PCR entwickelt. Ein Vergleich mit mikrobiologischen Daten zeigte, dass die Triplex-PCR Fusarium-Arten mit grosser Genauigkeit nachweisen kann. Es konnte eine positive Korrelation zwischen dem Auftreten eines PCR-Signals und dem Vorkommen des Mykotoxins Deoxynivalenol (DON) festgestellt werden.

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References

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Correspondence to S Knoll.

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Knoll, S., Niessen, L. & Vogel, R. Application of a PCR protocol for the diagnosis of trichothecene producingFusarium species in deoxynivalenol contaminated wheat. Mycotox Res 16, 240–243 (2000). https://doi.org/10.1007/BF02940048

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Keywords

  • Graminearum
  • Trichothecene
  • Fusarium Species
  • Deoxynivalenol
  • Wheat Sample