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The effects of castration and hormone replacement on runway behaviour and GABA concentrations in the septum


The correlation between changes in the concentration of brain biogenic amines and behaviour was assessed in groups of rats tested in a straight alleyway under food-rewarded conditions. Prior to behavioural assessment male rats were castrated, sham-operated or castrated with testosterone substitution. Behaviourally, animals were tested under continuous reinforcement or extinction schedules prior to killing and neurochemical assessment. Although no overall change in behaviour was found with castration, castrates showed a greater variability in day-to-day performance. Septal concentration of γ-aminobutyric acid (GABA) were decreased in the continuously-reinforced castrated group. Treatment of castrated rats with testosterone reversed this change in the septal concentration of GABA, and also showed a more stable runway performance. The variability in behaviour is considered in relation to the septal function.

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  1. Archer, J. 1974. The effects of testosterone on the distractability of chicks by irrelevant and relevant novel stimuli. Anim. Behav. 22, 397.

  2. Archer, J. 1977. Testosterone and persistence in mice. Anim. Behav. 25, 479.

  3. Caplan, M. 1973. An analysis of the effects of septal lesions on negatively reinforced behavior. Behav. Biol. 9, 129.

  4. Earley, C. J. and Leonard, B. E. 1976. The effects of castration on the neurochemical, behavioural and physiological correlates of differential food reward schedules in the alleyway. Irish J. Med. Sci. 145, 311.

  5. Earley, C. J. and Leonard, B. E. 1979. Consequences of reward and non-reward conditions: runway behavior, neurotransmitters and physiological indicators of stress. Pharmac. Biochem. Behav. 11, 215.

  6. Earley, C. J. and Leonard, B. E. 1978. GABA and gonadal hormones. Brain Research, 155, 27.

  7. Ellen, P. and Butter, J. 1969. External cue control of DRL performance in rats with septal lesions. Physiol. Behav. 4, 1.

  8. Ellen, P. and Kelnhofer, M. 1971. Discrimination of response feedback following septal lesions. Psychon. Sci. 23, 94.

  9. Gray, J. A., Rickwood, L., Drewett, R. F. and Dunne, E. 1977. Gonadal hormones and effects of partial reinforcement on appetitive behavior in the rat. Physiol. Behav. 19, 41.

  10. Thomas, J. B. 1972. Stimulus perseveration and choice behavior in rats with septal lesions. J. Comp. Physiol. Behav. 80, 97.

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Earley, C.J., Leonard, B.E. The effects of castration and hormone replacement on runway behaviour and GABA concentrations in the septum. I.J.M.S. 148, 227–231 (1979).

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  • Testosterone
  • Septal Lesion
  • Gonadal Hormone
  • Gaba Concentration
  • Cast Group