Springer Nature is making SARS-CoV-2 and COVID-19 research free. View research | View latest news | Sign up for updates

Comparison between a biochemical and a histochemical method for the detection of oestrogen receptor in breast carcinoma

  • 10 Accesses

  • 2 Citations

Summary

A histochemical method for the detection of oestrogen receptors in breast carcinoma is used to examine 48 cases of primary breast cancer and a comparison made with the standard biochemical method. Positive correlation between the two methods is shown in 85.4% of cases, a result which is statistically significant (p<0.02). Some points of advantage and disadvantage in both methods are discussed. In particular, the future role of histochemical methods in general, and of the labelled-ligand method of Lee, specifically, is considered. Although no definite clinical correlation between the histochemical method and patient management has yet been described, it is felt that the direct visualisation of oestrogen binding sites in breast cancer is a useful adjunct to the standard biochemical technique.

This is a preview of subscription content, log in to check access.

References

  1. Beatson, G. T. 1896. On the treatment of inoperable cases of carcinoma of the mamma. Lancet ii, 104–107.

  2. Clark, G. M., McGuire, W. L, Hubay, C. A. 1983. Progesterone receptors as a prognostic factor in Stage II breast cancer. N. Eng. J. Med. 309, 1343–1347.

  3. Fisher, B., Redmond, C. Brown, A. 1981. Treatment of primary breast cancer with chemotherapy and Tamoxifen. N, Eng. J. Med. 305, 1–6.

  4. Furnival, C. M., Chong, K. C. Watson, L. R. and Visona, A. 1983. Fluorescent cytochemical oestrogen receptor assay: is it valid in breast cancer? Brit. J. Surg. 70, 457–459.

  5. Hanna, W., Ryder, D. E. and Mobbs, B. G. 1982. Cellular localisation of oestrogen binding sites in human breast cancer. Am. J. Clin. Pathol. 77, 391–395.

  6. Kiang, D. T., Frennine, D. H., Goldman, A. I. 1978. Oestrogen receptors and responses to chemotherapy and hormonal therapy in advanced breast cancer. N. Eng. J. Med. 299, 1330–1334.

  7. King, W. J. and Greene, G. I. 1984. Monoclonal antibodies localize oestrogen receptor in the nuclei of target cells. Nature 37, 745–747.

  8. Knight, W. Q., Livingston, R. B., Gregory, E. J. and McGuire, W. L. 1977. Oestrogen receptor as an independent prognostic factor for early recurrence in breast cancer. Cancer Res. 37, 669–4671.

  9. Lancet. 1984. i, pp. 887–888. Steroid receptors and prognosis of breast cancer.

  10. Lee, S. H. 1978. Cytological study of oestrogen in human mammary cancer. Am. J. Clin. Pathol. 197–203.

  11. Lee, S. H. 1980. Cellular oestrogen and progesterone receptors in mammary carcinoma. Am. J. Clin. Pathol. 73, 323–329.

  12. Lee, S. H. 1984. Validity of a histochemical oestrogen receptor assay supported by the observation of a cellular response to steroid manipulation. J. Histochem. Cytochem. 32, 305–310.

  13. Lesser, M. L., Rosen, P. P., Senie, R. T. 1981. Oestrogen and progesterone receptors in breast carcinoma: correlations with epidemiology and pathology. Cancer 48, 299–309.

  14. Lippman, M. E., Allegra, J. C. Thompson, E. B. 1978. The relation between oestrogen receptors and response rate to cytochemical chemotherapy in metastatic breast cancer. N. Eng. J. Med. 298, 1223–1228.

  15. Lippman, M. E. and Allegra, J. C. 1980. Quantitative oestrogen receptor analyses. The response to endocrine and cytotoxic chemotherapy in human breast cancer and the disease-free interval. Cancer 46, 2829–2834.

  16. McCarthy, K. S. Jr., Woodward, B. H., Nicholas, D. E., Wilkinson, W. and McCarthy, K. S. Sr. 1980. Comparison of biochemical and histochemical techniques for oestrogen receptor analysis in mammary carcinoma. Cancer 46, 2842–2845.

  17. Meijer, C. J. L. M., Van Marie, J., Persijn, J. P. et al. 1982. Oestrogen receptors in human breast cancer. II. Correlation between the histochemical method and the biochemical assay. Virchows Arch. (Cell. Pathol.) 40, 27–37.

  18. Meyer, J. S., Stevens, S. C., White, W. L. and Hixon, B. 1978. Oestrogen receptor assay of carcinomas of the breast by a simplified Dex-tran-Charcoal method. Am. J. Clin. Pathol. 70, 644–655.

  19. Millis, R. R. 1980. Correlation of hormone receptors with pathological features in human breast cancer. Cancer 46, 2869–2871.

  20. Panko, W. B., Watson, C. S. and Clark, J. H. 1981. The presence of a second specific oestrogen binding site in human breast cancer. J. Steroid Biochem. 14, 1311–1316.

  21. Panko, W. B., Mattioli, C. A. and Sheeler, T. M. 1982. Lack of correlation of histochemical method for oestrogen receptor analysis, the biochemical assay results. Cancer 49, 2148–2152.

  22. Pertschuk, L P. 1978. Immunofluorescent detection of oestrogen receptor in breast cancer. Cancer 41, 907–911.

  23. Pertschuk, P. P., Gaetjens, E., Carter, A., Brigati, D. J., Kim, D. S. and Fealey, T. E. 1979. An improved histochemical method for detection of oestrogen receptors in mammary cancer. Am. J. Clin. Pathol. 71, 504–508.

  24. Pertschuk, L. P., Eisenberg, K. B., Leo, V. V., Carter, A. C. and Gaetjens, E. 1982. Correlation of oestrogen binding histochemistry in breast cancer with clinical response to hormonal therapies (Abstract). Lab. Invest. 46, p65A.

  25. Stumpf, W. E. 1983. The histochemistry of steroid hormone receptors. J. Histochem Cytochem. 31, 113–114.

  26. Underwood, W. E. 1983. Oestrogen receptors in human breast cancer: review of histopathological correlations and critique of histochemical methods. Diagnostic histopathology 6, 1–22.

  27. Van Marle, J., Lindeman, J., Ariens, A. Th., Labruyere, W. and Van Weeran-Kramer, J. 1982. Oestrogen receptors in human breast cancer. I. Specificity of the histochemical localisation of oestrogen receptors using an oestrogen-albumin FITC complex. Virchows Arch (Cell. Pathol.) 40, 1–25.

  28. Walker, R. A., Cove, D. H. and Hewell, A. 1980. Histological detection of oestrogen receptor in human breast carcinomas. Lancet i, 171–173.

  29. Yao, X., Meng, X., Chen, P. and Mei, Z. 1983. Histochemical receptor assay for selecting stage III breast cancers for combined chemohormonal therapy (Abstract). Lab. Invest. 48, p95A.

Download references

Author information

Correspondence to R. T. McMahon or C. E. Connolly.

Additional information

Charles Eugene Connolly wishes to acknowledge a generous grant from the Irish Cancer Society, without which the biochemical assay could not have been carried out.

Rights and permissions

Reprints and Permissions

About this article

Cite this article

McMahon, R.T., Connolly, C.E. Comparison between a biochemical and a histochemical method for the detection of oestrogen receptor in breast carcinoma. I.J.M.S. 154, 187–192 (1985). https://doi.org/10.1007/BF02937359

Download citation

Keywords

  • Breast Cancer
  • Oestrogen Receptor
  • Progesterone Receptor
  • Human Breast Cancer
  • Biochemical Method