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Investigation of malignancy-related glycopeptides and their releasein vitro andin vivo from ascites tumour cells

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LANDSCHÜTZ mouse ascites tumour cells contain trypsin-sensitive fucopeptides similar to those known in other tumour cell types to be closely related to malignancy. These components were found not to be released from the cellsIn vitro unless trypsin was present. They were, however, spontaneously shed from the tumourin vivo into ascitic fluid within 24 h. Comparison of cellular and released fucopeptides showed that the components releasedin vitro andin vivo represented a specific population of cellular fucopeptides. The action of trypsin in releasing these entitiesin vitro was found to be the sum of several small changes involving various fucopeptide species, mainly of intermediate size and charge. Trypsin-inducedin vitro release and spontaneous releasein vivo involved slightly different proportions of individual fucopeptide species.

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Correspondence to E. Walsh or H. Smyth or A. Corrigan.

Additional information

We are grateful to The Medical Research council of Ireland for financial support for this project, Dr. M. L. Conalty and staff of the M.R.C. Laboratories, trinity Colletge, Dublin for supplying tumour mice, and Drs. R. O’Kennedy and M. Clynes, N.I.H.E.,Dublin for helpful comments.

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Walsh, E., Smyth, H. & Corrigan, A. Investigation of malignancy-related glycopeptides and their releasein vitro andin vivo from ascites tumour cells. I.J.M.S. 154, 292–305 (1985). https://doi.org/10.1007/BF02937168

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  • Sialic Acid
  • Hexose
  • Glycopeptide
  • Fucose
  • Ascitic Fluid