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Immobilization ofThiobacillus Denitrificans for the oxidation of hydrogen sulfide in sour water

Abstract

It has been demonstrated that viable cells of the chemoautotroph and facultative anaerobeThiobacillus denitrificans can be used to remove soluble sulfides from sour water with anaerobic oxidation to sulfate. A packed bed of immobilized T.denitrificans was capable of removing all traces of sulfide from an aqueous feed stream containing 26 ppm sulfide during a continuous 12 day test. Calcium carbonate provided buffering capacity and generated Ca+2 internal to alginate beads to maintain structural integrity during extended periods of operation.

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References

  1. 1.

    Postgate, J. R. (1984),The Sulfate-Reducing Bacteria, 2nd ed., Cambridge University Press, Cambridge.

  2. 2.

    Hamilton, W. A. (1985),Ann. Rev. Microbiol. 39, 195.

  3. 3.

    Ruseska, I., Robbins, J., and Costerton, J. W. (1982),Oil Gas J. p. 253.

  4. 4.

    National Association of Corrosion Engineers (1976),The Role of Bacteria in the Corrosion of Oil Field Equipment, NACE, Houston, TX.

  5. 5.

    Noyes Data Corp. (1973),Pollution Control in the Petroleum Industry, Noyes Data Corp., Park Ridge, NJ.

  6. 6.

    Baalsrud, K. and Baalsrud, K. S. (1954),Archiv. Mikrobiol. 20, 34.

  7. 7.

    Sublette, K. L. and Sylvester, N. D. (1987),Biotech. Bioeng. 29, 249.

  8. 8.

    Sublette, K. L. (1987),Biotech Bioeng. 29, 690.

  9. 9.

    Sublette, K. L. and Sylvester, N. D. (1987),Biotech. Bioeng. 29, 753.

  10. 10.

    Sublette, K. L. and Sylvester, N. D. (1987),Biotech. Bioeng. 29, 759.

  11. 11.

    Lewandowski, Z., Bakke, R., and Characklis, W. G. (1987),Wat. Sci. Tech. 19, 175.

  12. 12.

    American Public Health Association (1976),Standard Methods for Examination of Water and Wastewater, 14th ed., APHA, New York (1976).

  13. 13.

    Meites, L., ed. (1963),Handbook of Analytical Chemistry, McGraw-Hill, New York.

  14. 14.

    Truper, H. G. and Schlegel, H. G. (1964),Ant. van Leeuwenhoek 30, 225.

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Author information

Correspondence to Kerry L. Sublette.

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Sublette, K.L. Immobilization ofThiobacillus Denitrificans for the oxidation of hydrogen sulfide in sour water. Appl Biochem Biotechnol 20, 675–686 (1989). https://doi.org/10.1007/BF02936516

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Index Entries

  • Hydrogen sulfide
  • Thiobacillus denitrificans
  • sour water
  • immobilization
  • packed-bed