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Effect of arsenic trioxide on apoptosis of pulmonary eosinophile in asthmatic Guinea-Pigs

Abstract

Objective

To study the effect and mechanism of arsenic trioxide (As2O3) on apoptosis of pulmonary eosinophiles (PE) in asthmatic guinea-pigs.

Methods

Thirty guinea-pigs were divided into 3 groups at random, the control group, the asthmatic group and the As2O3 treated group. The dosage of As2O3 used was 2 mg/kg. The apoptotic PE were labelled by TdT-mediated dUTP nick end labelling technique, and the PE infiltration and apoptosis were detected quantitatively by using computerized image analysis technique.

Results

In the control group, the amount of infiltrating PE was 4.4±2.5 cells/HP and the PE apoptotic index (AI) was 0.42±0.08%. In the asthmatic group, the amount of inflitrating PE increased (P<0.01) and AI decreased significantly (P<0.01). After the asthmatic animals had been treated with As2O3, the two parameters changed reversedly significantly (P<0.01), and there was a significantly negative correlation between them ( r = — 0.949,P<0.01).

Conclusion

The PE apoptosis abnormality is one of the important mechanisms that cause bronchial asthma, As2O3 could alleviate the airway inflammation through promoting PE apoptosis and lower PE infiltration. Low dose of As2O3 is proved to be effective with relative safety, it also has potential value in treating asthma.

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Author information

Correspondence to Lin-fu Zhou or Kai-sheng Yin.

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Cite this article

Zhou, L., Yin, K. Effect of arsenic trioxide on apoptosis of pulmonary eosinophile in asthmatic Guinea-Pigs. CJIM 8, 104 (2002). https://doi.org/10.1007/BF02934433

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Keywords

  • As2O3
  • Apoptotic Index
  • Venous Sinus
  • Arsenic Trioxide
  • Image Analysis Technique