Objective: To study the inhibitory effect of purified trichosanthin component on the proliferation of malignant melanoma.Methods: The effect of purified trichosanthin component on the DNA synthesis, cell cycle and cell apoptosis of murine melanoma cells were detected by flow cytometry when cultured in vitro.Results: The significant G0/G1 phase arrest was revealed by the increase of cells in G0/G1 phase and decrease of cells in S phase. The obvious apoptosis of melanoma cells was induced by purified trichosanthin component. G0/ G1 phase arrest was highly correlated with apoptosis (r = 0. 8705).Conclusion: The purified trichosanthin component can markedly inhibit melanoma cells by the suppression of DNA synthesis in S phase and cell mitosis as well as induction of cell apoptosis.
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Mayer RA, Sergios PA, Coonan K, et al. Trichosanthin treatment of HIV-induced immune dysregulation. Eur J Clin Invest 1992; 22(2): 113–122.
Wang QC, Ying WB, Xie H, et al. Trichosanthin-monoclonal antibody conjugate specially cytotoxic to human hepatoma cells in vitro. Cancer Res 1991; 51(13): 3353–3355.
Carson DA, Ribeiro JM. Apoptosis and disease. Lancet 1993; 341(15): 1251–1254.
Song Q, Baxter GD, Kovacs EM, et al. Inhibition of apoptosis in human tumor cells by okadaic acid. J Cell Physiol 1992; 153(3): 550–556.
1994; 14(1): 18–20.
Supported by the fund from National Natural Science Foundation of China (approved No. 39770913)
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Liqi, B., Hongjun, L. & Deming, Z. Effect of trichosanthin on cell cycle and apoptosis of murine melanoma cells. CJIM 5, 63–65 (1999). https://doi.org/10.1007/BF02934194
- cell cycle