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Effect of trichosanthin on cell cycle and apoptosis of murine melanoma cells

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Abstract

Objective: To study the inhibitory effect of purified trichosanthin component on the proliferation of malignant melanoma.Methods: The effect of purified trichosanthin component on the DNA synthesis, cell cycle and cell apoptosis of murine melanoma cells were detected by flow cytometry when cultured in vitro.Results: The significant G0/G1 phase arrest was revealed by the increase of cells in G0/G1 phase and decrease of cells in S phase. The obvious apoptosis of melanoma cells was induced by purified trichosanthin component. G0/ G1 phase arrest was highly correlated with apoptosis (r = 0. 8705).Conclusion: The purified trichosanthin component can markedly inhibit melanoma cells by the suppression of DNA synthesis in S phase and cell mitosis as well as induction of cell apoptosis.

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Author information

Correspondence to Bi Liqi or Li Hongjun or Zheng Deming.

Additional information

Supported by the fund from National Natural Science Foundation of China (approved No. 39770913)

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Liqi, B., Hongjun, L. & Deming, Z. Effect of trichosanthin on cell cycle and apoptosis of murine melanoma cells. CJIM 5, 63–65 (1999). https://doi.org/10.1007/BF02934194

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Key Words

  • trichosanthin
  • melanoma
  • apoptosis
  • cell cycle
  • flowcytometry