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Effect of culture conditions on monoclonal antibody production from genetically modified tobacco suspension cultures


Oxygen supply and inoculum age were found to affect the production of the heavy chain monoclonal antibody (HC MAb) from genetically modified tobacco suspension cultures. The increase of oxygen supply increased both cell growth and HC MAb production. Furthermore, the increased aeration and mixing improved the production of HC MAb based on the unit amount of cells or total soluble proteins. This indicated that the increased aeration improved the production and secretion of HC MAb more than other cell components. HC MAb production and cell growth also improved when batch cultures were inoculated with actively dividing cells (5-day old) rather than the fullygrown cells (7- or 10-day old cells) that are commonly used for subcultures. The addition of glutamine to the medium also improved cell growth and HC MAb production.

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Correspondence to James M. Lee.

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Liu, F., Lee, J.M. Effect of culture conditions on monoclonal antibody production from genetically modified tobacco suspension cultures. Biotechnol. Bioprocess Eng. 4, 259–263 (1999).

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Key words

  • monoclonal antibody
  • tobacco suspension cultures
  • oxygen supply
  • inoculum age
  • glutamine addition