Pharmaceutisch weekblad

, Volume 1, Issue 1, pp 1205–1213 | Cite as

Preparation of lyophilised drug spiked calf's serum

  • I. C. Dijkhuis
  • E. W. De Flines
  • J. H. Rengerink


In this study, a survey is given of the preparation of lyophilised antiepileptic quality control serum specimens, as used in the joint international test on antiepileptic drugs (Dijkhuiset al. 1979).

Calfs serum, previously filtered through cellulose asbestos pads was used as a matrix and spiked with 8 antiepileptic drugs; the drug concentrations were calculated on basis of weighed-in values, corrected for increase of volume after reconstitution.

Difficulties, encountered by components, eluted from rubber andPvc tubes and stoppers during filtration and lyophilisation procedures are discussed.

As an example, the preparation of a batch of lyophilised quality control serum is described in detail.

A preliminary report is given on serum filtration using the Pellicon Cassette Ultrafiltration System, developed by Millipore. (Pharm. Weekblad Sci. Ed.1, 185-196)


Valproic Acid Antiepileptic Drug Serum Specimen Ethosuximide Serum Drug Concentration 
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  1. Burger, H., andJ. Polderman (1965) Apparatuur voor de bestudering van het droogvriesproces,Pharm. Weekblad 100, 1125–1133.Google Scholar
  2. Deluca, P., andL. Lachman (1965) Lyophilization of Pharmaceuticals. 1. Effect on certain physical-chemical properties,J. Pharm. Sci. 54, 617–624.CrossRefPubMedGoogle Scholar
  3. Dijkhuis, I. C. (1979)Quality control of drug assays in therapy and toxicology, using calf serum as a matrix. Thesis, Leiden.Google Scholar
  4. Dukhuis, I. C., H. J. De Jong, A. Richens, C. E. Pippenger, E. E. A. Leskinen andA. P. W. Nyberg (1979) Joint international quality control programme on the determination of antiepileptic drugs.Pharm. Weekblad Sci. Ed. 1, 151–184.Google Scholar
  5. Uges, D. R. A., andP. Bouma (1978) An improved determination of clonazepam in serum byHplc,Pharm. Weekblad 113, 1156–1158.Google Scholar
  6. Wallace, J. E., J. K. Farguhar, H. E. Hamilton andB. A. Everhart (1974) Comments on the determination of diphenylhydantoin,Clin. Chem. 20, 515–516.PubMedGoogle Scholar
  7. Wilensky, A. J. (1978) Stability of some antiepileptic drugs in plasma,Clin. Chem. 24, 722.PubMedGoogle Scholar

Copyright information

© Bohn, Scheltema & Holkema 1979

Authors and Affiliations

  • I. C. Dijkhuis
    • 1
  • E. W. De Flines
    • 1
  • J. H. Rengerink
    • 1
  1. 1.The Hague Hospitals Central PharmacyThe HagueThe Netherlands

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