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Pharmaceutisch weekblad

, Volume 1, Issue 1, pp 769–774 | Cite as

Macro and micro approaches to the determination of agricultural poisons in human and animal tissues

  • R. A. A. Maes
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Abstract

Analytical approaches to the determination of agricultural poisons and their metabolites in human and animal tissues will take many forms. Several factors have to be considered in choosing an analytical scheme if the results are to be meaningful. Whenever possible the toxicologist will use standardised methodology, which has been subjected to evaluation by collaborative studies. In the absence of such methods, the analyst must be aware of the capabilities and limitations of the various steps in the analytical procedure of choice. Extraction and cleanup efficiency must be considered in view of the determinative method to be used. Highly efficient gas Chromatographic columns, coupled with sensitive and selective detector systems, are powerful analytical tools. One must be cautious, however, when relying on gas Chromatographic peak retention time for compound identification. For a complete analysis, confirmatory techniques should be used. The available sample size may dictate the use of a micro method of analysis and, at the same time, preclude certain confirmatory techniques. Advantages of micro methods include: speed, smaller glassware and minimal solvent volumes.

Keywords

Pesticide Residue Heptachlor Heptachlor Epoxide Large Column Florisil Column 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Samenvatting

Vele analytische methoden voor de bepaling van landbouwvergiften en hun metabolieten in humane en dierlijke weefsels zijn mogelijk. Voor zinvolle resultaten moet men bij het kiezen van een analyseschema verschillende factoren in beschouwing nemen. De toxicoloog zal, indien enigszins mogelijk, standaardmethodieken gebruiken die door gezamenlijke studies op hun waarde zijn getoetst. Bij ontbreken van zulke methoden moet de analyticus zich bewust zijn van de mogelijkheden en de beperkingen van de verschillende stappen in de analyseprocedure van de bepaling van zijn keus. Extractie en ‘de meest praktische’ zuiveringsmethode moeten worden beschouwd in relatie tot de toe te passen bepalingsmethode. Uiterst efficiënte gaschromatografie-kolommen in combinatie met gevoelige en selectieve detectiesystemen zijn goede analysehulpmiddelen. Men moet echter oppassen met het te veel vertrouwen van de gaschromatografische piek-retentietijden voor identificatie van verbindingen. Voor een volledige analyse moet men betrouwbare identificatietechnieken toepassen. De beschikbare hoeveelheid monster kan het gebruik van een micro-analysemethode noodzakelijk maken en tegelijkertijd bepaalde technieken van identificatie uitsluiten. Voordelen van micromethoden zijn onder meer: snelheid, kleiner glaswerk en minimale hoeveelheden oplosmiddelen.

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References

  1. Erney, D. R. (1974) A feasibility study of miniature silica gel columns for the separation of some polychlorinated biphenyls,Ddt, and analogs,Bull. Environm. Contam. Toxicol. 12, 710.CrossRefGoogle Scholar
  2. Griffitt, K. R., andJ. C. Craun (1974) Gel permeation Chromatographic system: an evaluation,J. Am. Offic. Anal. Chem. 57, 168.Google Scholar
  3. Holden, A. V., andK. Marsden (1969) Single-stage cleanup of animal tissue extracts for organochlorine residue analysis,J. Chromatog. 44, 481.CrossRefGoogle Scholar
  4. O'keefe, P. W. (1978) A neutral cleanup procedure forTcdd residues in environmental samples. In:Dioxin, toxicological and chemical aspects (Ed.F. Cattabeni, A. Cavallaro andG. Galli). sp Medical and Scientific Books, New York-London, p. 59.Google Scholar
  5. Mills, P. A. (1959) Detection and semi-quantitative estimation of chlorinated organic pesticides residues in foods by paper chromatography,J. Am. Offic. Anal. Chem. 42, 734.Google Scholar
  6. Mills, P. A., B. A. Bong, L. R. Kamps andJ. A. Burke (1972) Elution solvent system for Florisil column cleanup in organochlorine pesticides residue analyses,J. Am. Offic. Anal. Chem. 55, 39.Google Scholar
  7. Mills, P. A., J. H. Onley andR. A. Gathier (1963) Rapid method for chlorinated pesticides residues in non-fatty foods,J. Am. Offic. Anal. Chem. 46, 186.Google Scholar
  8. Ryan, J. F. (1977) Residue analysis applications of Mass spectrometry. In:Analytical methods for pesticides and plant growth regulators (Ed.G. Zweig). Academic Press, New York — San Francisco — London.Google Scholar
  9. Sherma, J., andT. M. Shafik (1975) A multiclass, multiresidue analytical method for determining pesticide residues in air,Arch. Environm. Contain. Toxicol. 3, 55.CrossRefGoogle Scholar
  10. Stalling, D. L., R. C. Tindle andJ. L. Johnson (1972) Cleanup of pesticides and poly chlorinated biphenyl residues in fish extracts by gel permeation chromatography,J. Am. Offic. Anal. Chem. 55, 32.Google Scholar
  11. Storherr, R. W., E. J. Murray, I. Klein andL. A. Rosenburg (1967) Sweep co-distillation cleanup of fortified edible oils for determination of organophosphate and chlorinated hydrocarbon pesticides,J. Am. Offic. Anal. Chem. 50, 605.Google Scholar
  12. Thompson, J. F., S. J. Reid andE. J. Kantor (1977) A multiclass, multiresidue analytical method for pesticides in water,Arch. Environm. Contam. Toxicol. 6, 143.CrossRefGoogle Scholar

Copyright information

© Bohn, Scheltema & Holkema 1979

Authors and Affiliations

  • R. A. A. Maes
    • 1
  1. 1.Center for Human ToxicologyState UniversityUtrecht

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