Pharmaceutisch Weekblad

, Volume 2, Issue 1, pp 1105–1109 | Cite as

Assay of propranolol in human and rat plasma by capillary gas chromatography with nitrogen selective detection

  • A. G. De Boer
  • D. D. Breimer
  • J. M. Gubbens-Stibbe
Original Article


A gas Chromatographic method is described for the assay of propranolol in human and rat plasma. The extraction with diethylether is relatively simple compared to existing procedures and there is no need for clean-up. Following derivatisation with acetic acid anhydride the derivatives were determined by means of capillary gas chromatography, solid injection and nitrogen-phosphorous selective detection.

Calibration curves were linear in the concentration range from 5 to 100 ng/ml plasma. The recovery of propranolol from human and rat plasma was 80% and 100% respectively while the detection limit in both cases was approximately 1 ng/ml. There is no interference with endogenous plasma substances or metabolites and retention times are short (ca. 3 min). An application has been given. (Pharm. Weekblad Sci. Ed.2, 101–105).


Nitrogen Acetic Acetic Acid Detection Limit Retention Time 
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Copyright information

© Bohn, Scheltema & Holkema 1980

Authors and Affiliations

  • A. G. De Boer
    • 1
  • D. D. Breimer
    • 1
  • J. M. Gubbens-Stibbe
    • 1
  1. 1.Department of Pharmacology, Subfaculty of PharmacyUniversity of LeidenLeidenThe Netherlands

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